Oligo Purification

Oligo Reconstitution

Primer Design

Long Oligos

Modifications

Sample Oligo Report

Affinity Ligands & Enzyme Conjugates

Oligonucletides can be labeled with biotin or digoxigenin* or directly labeled with alkaline phosphatase. A variety of linker arms are available as spacers to minimize steric hindrance.

Besides their importance as nucleic acid probes, biotinylated oligonucleotides are also useful for the purification of DNA binding proteins or cognate DNA molecules by specific hybridization based affinity chromatography. The biotinylated oligonucleotide can be bound to a streptavidin matrix and used for either column or spin chromatography.

Modifications for Oligo Synthesis
Product	Catalog No.	50 nmol scale (XX=05)	200 nmol scale (XX=02)	1 mmol scale (XX=01)	10 mmol scale (XX=10)	15 mmol scale (XX=15)
Biotin dT	26-64XX-XX	220.00	220.00	310.00	Inquire	Inquire
5’ or 3’ biotinylation	26-64XX-XX	90.00	90.00	190.00	Inquire	Inquire
Biotin-TEG	26-64XX-XX	250.00	250.00	350.00	Inquire	Inquire
Digoxigenin	26-64XX-XX	350.00	350.00	450.00	Inquire	Inquire
Alkaline Phosphatase#	26-64XX-XX	550.00	550.00	1200.00	Inquire	Inquire

* Roche holds exclusive rights to digoxigenin labeling. Digoxigenin oligo labeling is offered under license from Roche. Extensive digoxigenin labeling techniques and detection methods are available from Roche.