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BiotinTEG Azide

picture of BiotinTEG Azide

Modification : BiotinTEG Azide

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6721
Click Chemistry
Bio-TEG-N3
Y
Y
Y
444.55
-
PS26-6721.pdf
-
-
-


Catalog NoScalePrice
26-6721-0550 nmol$450.00
26-6721-02200 nmol$450.00
26-6721-011 umol$585.00
26-6721-032 umol$877.50
26-6721-1010 umol$4,680.00
26-6721-1515 umol$5,850.00

Biotin-TEG Azide is a biotin attached to a 15-atom mixed polarity triethylene glycol spacer with an azide group at the end of the spacer. The presence of the azide allows the user to use Click Chemistry (a [3+2] cycloaddition reaction between alkynes and azides, using copper (I) iodide as a catalyst) to conjugate the Biotin-TEG Azide to a terminal alkyne-modified oligo with extremely high regioselectivity and efficiency (1,2). Preparation of the alkyne-modified oligo can be achieved using the 5-Hexynyl modifier (see its respective tech sheet for details). The spacer acts to minimize steric hindrance between the biotin moiety and the oligo, thereby providing streptavidin easy access to the biotin for capture and immobilization of the oligo. Additional technical details for biotin are presented in the Biotin technical sheet.

References
1. Huisgen, R. Angew. Chem. Int. Ed. (1963), 2: 565-568.
2. Rostovtsev, V.V., Green, L.G., Fokin, V.V., Sharpless, K.B. A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Regioselective Ligation of Azides and Terminal Alkynes. Angew. Chem. Int. Ed. (2002), 41: 2596-2599.
- BiotinTEG Azide

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