Oligo Purification

Oligo Reconstitution

Primer Design

Long Oligos

Modifications

Sample Oligo Report

Price Lists for Oligonucleotides

Unmodified Oligonucleotides (price per base)
Product	50 nmol scale	200 nmol scale	1 mmol scale	2 mmol scale	10 mmol scale	15 mmol scale
Unmodified Oligonucleotides	$0.90	$2.00	$3.75	$6.50	$32.00	$38.00
Same Day Oligo Service	$20.00 per oligo (Must order by 12 PM EST Time)

Oligo Scale of Synthesis and Typical Yield

Crude Desalted RPC Purified** Gel Purified 20 mer oligo Typical Yield 30 mer oligo Typical Yield 50 mer oligo Typical Yield Scale A260 Units nmols mg A260 Units nmols mg A260 Units nmols mg 50 nmol 8-10 30+ 0.2-0.3 4-5 12+ 0.1-0.16 NR* [1-2] NR* [2-4] NR* [0.03-0.06] 200 nmol 20-25 80+ 0.6-0.8 8-12 24+ 0.26-0.4 4-6 8+ 0.13-0.2 1 mmol 100-120 400+ 3-4 40-50 90+ 1.3-1.6 20-25 40+ 0.6-0.8 Purity & Yield Purity is greater than 80% depending on oligo sequence and structure. Refer to coupling efficiency table for oligo length dependent purity and yield. No further purification required for PCR and sequencing applications. Gel purification recommended for oligos above 50 mer and all applications involving cloning and mutagenesis. **RPC is reverse phase purification using a cartridge; a substitue for HPLC. Purity 85% to 95% depending on oligo sequence and structure Yield and Purity will be lower for sequences with high GC content Not recommended for oligos longer than 35 mer. **RPC is reverse phase purification using a cartridge; a substitue for HPLC. Purity 98% to ~100% depending on oligo sequence and structure Yield will gradually decrease as length of oligo increases. Palindromes, hairpins and high GC content oligos and oligos containing stretches of 3 or more G's induce strong secondary structure and base stacking thus decreasing purity and yield. *NR Not Recommended

*Yield of 30 µg/A260 unit for oligos is calculated for an ~equimolar base composition. Long stretches of a single base or homopolymers will have variable yields. Example for homopolymeric 50 mer: A(50) = ~20/A260 Unit; G(50) = ~28/A260 Unit; T(50) = ~35/A260 Unit and C(50) = ~39/A260 Unit.

Purification (price per oligo)
Product	Catalog No.	50 nmol scale	200 nmol scale	1 mmol scale	2 mmol scale	10 mmol scale	15 mmol scale
Gel Purification	26-6400-XX	75.00	75.00	150.00	280.00	1500.00	1800.00
Reverse Phase Cartridge	26-6400-XX	30.00	30.00	90.00	170.00	750.00	900.00