Oligo Purification

Oligo Reconstitution

Primer Design

Long Oligos

Modifications

Sample Oligo Report

Introduction Gene Link Oligo Synthesis Division is not an "oligo factory". Order Gene Link Oligos for demanding applications and consistent results. Gene Link oligos will not fail due to quality reasons. Each oligo is meticulously monitored during synthesis for coupling efficiency and after processing by polyacrylamide gel electrophoresis. A Gel picture is supplied with each oligo. All modifications available Molecular Beacons, TaqMan Probes, Fluorescent Dye labeled oligos and probes RNA and 2'O methyl oligos, including chimeras Antisense oligos, phosphorothioate oligos Long oligo synthesis, up to 250 mer Easy online ordering New Customers buy one, get one free. Any size up to 250 mer. All Gene Link synthesized custom oligonucleotides are supplied desalted, lyophilized and ready to use after reconstruction. The oligonucleotides are synthesized on state of the art computer controlled DNA synthesizers. The "crude" desalted ethanol precipitated product is suitable for most PCR** amplification, Short Tandem Repeat Polymorphism (STRP) repeat analysis for gene mapping and sequencing experiments. An actual gel picture of quality assurance polyacrylamide gel electrophoresis and technical information e.g. Tm, mw, base composition and the exact A 260 units supplied.

Same Day Oligo Service

Certain experiments cannot wait for an extra day. When such is the case, order our Same Day Oligo service. The custom oligo will be shipped the same day for next day am or pm delivery. Order should be received before 12 noon; this service is for oligos shorter than 30mer and will be synthesized only on a 200nmol scale. Gel picture and A 260 will not be provided. Gel picture will be mailed if requested. Confirmation required.

Same Day Oligo Service

$20.00 per oligo (Must order by 12 PM EST Time)

Oligo Scale of Synthesis and Typical Yield

Crude Desalted RPC Purified** Gel Purified 20 mer oligo Typical Yield 30 mer oligo Typical Yield 50 mer oligo Typical Yield Scale A260 Units nmols mg A260 Units nmols mg A260 Units nmols mg 50 nmol 8-10 30+ 0.2-0.3 4-5 12+ 0.1-0.16 NR* [1-2] NR* [2-4] NR* [0.03-0.06] 200 nmol 20-25 80+ 0.6-0.8 8-12 24+ 0.26-0.4 4-6 8+ 0.13-0.2 1 mmol 100-120 400+ 3-4 40-50 90+ 1.3-1.6 20-25 40+ 0.6-0.8 Purity & Yield Purity is greater than 80% depending on oligo sequence and structure. Refer to coupling efficiency table for oligo length dependent purity and yield. No further purification required for PCR and sequencing applications. Gel purification recommended for oligos above 50 mer and all applications involving cloning and mutagenesis. **RPC is reverse phase purification using a cartridge; a substitue for HPLC. Purity 85% to 95% depending on oligo sequence and structure Yield and Purity will be lower for sequences with high GC content Not recommended for oligos longer than 35 mer. **RPC is reverse phase purification using a cartridge; a substitue for HPLC. Purity 98% to ~100% depending on oligo sequence and structure Yield will gradually decrease as length of oligo increases. Palindromes, hairpins and high GC content oligos and oligos containing stretches of 3 or more G's induce strong secondary structure and base stacking thus decreasing purity and yield. *NR Not Recommended