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| TaqMan probes with Tamra at 3' end and Molecular beacons with universal fluorescence quencher dabcyl or BHQ at 3’ end. Price includes gel purification. Gene Link considers gel purification to be the best method of purification and essential for optimum performance of fluorescent dye labeled oligonucleotides.Customers may request Molecular Beacons without gel purification for reduced pricing. |
| Fluorophore* | Color | Absorbance max (nm) | Emission max (nm) | $, 200 nmol scale | $, 1 mmol scale |
| Dabcyl (Quencher) | 453 | $90 | $150 | ||
| BHQ-1** (Quencher) | 534 | $90 | $150 | ||
| BHQ- 2** (Quencher) | 579 | $90 | $150 | ||
| BHQ-3** (Quencher) | 672 | $90 | $150 | ||
| 6-FAM (Fluorescein) | Green | 494 | 525 | $290 | $390 |
| TET | Orange | 521 | 536 | $290 | $390 |
| HEX | Pink | 535 | 556 | $290 | $390 |
| Cy 3 | Red | 552 | 570 | $450 | $590 |
| Cy 3.5 | Purple | 588 | 604 | $540 | $690 |
| Cy 5 | Violet | 646 | 667 | $450 | $590 |
| Cy 5.5 | Blue | 683 | 707 | $540 | $690 |
| Cy 7 | Near IR | 743 | 767 | $540 | $690 |
| Tetramethylrhodamine | Rose | 565 | 580 | $540 | $690 |
| ROX | Purple | 587 | 607 | $540 | $690 |
| JOE | Mustard | 528 | 554 | $540 | $690 |
| Volume and contract discount pricing available. Pricing subject to change without notice. | |||||
| Fluorescent Probes Price List Summary* and Guaranteed Yield** | ||||||
| 50 nmol scale | 200 nmol scale | 1 mmol scale | ||||
| Dye Quencher | Price | Yield | Price | Yield | Price | Yield |
| 5' 6-FAM, HEX or TET, 3' Tamra,BHQ or Dabcyl | $220.00 | 10 nmol | $290.00 | 20 nmol | $390.00 | 40 nmol |
| 5'-Cy 3 or Cy 5/BHQ2 | $290.00 | 5 nmol | $450.00 | 10 nmol | $590.00 | 20 nmol |
| 5' NHS (ROX, Texas Red, Alexa, Cy3.5, 5.5 & 7) | Not Available | $540.00 | 8 nmol | $690.00 | 16 nmol | |
| *Price includes purification. Royalty charges are additional. Pricing subject to change without notice. | ||||||
| **Guaranteed yield for dye and quencher listed. Lower yield with additional modifications. | ||||||
| Alexa Dye Series | ||||
| Alexa Fluor Dye | Color | Absorbance max (nm) | Emission max (nm) | Extinction Coefficient |
| Alexa Fluor 350 | 346 | 442 | 19,000 | |
| Alexa Fluor 405 | 401 | 421 | 34,000 | |
| Alexa Fluor 430 | 433 | 541 | 16,000 | |
| Alexa Fluor 488 | 495 | 519 | 71,000 | |
| Alexa Fluor 532 | 532 | 553 | 81,000 | |
| Alexa Fluor 546 | 556 | 573 | 104,000 | |
| Alexa Fluor 555 | 555 | 565 | 150,000 | |
| Alexa Fluor 568 | 578 | 603 | 91,000 | |
| Alexa Fluor 594 | 590 | 617 | 73,000 | |
| Alexa Fluor 633 | 632 | 647* | 100,000 | |
| Alexa Fluor 647 | 650 | 665* | 239,000 | |
| Alexa Fluor 660 | 663 | 690* | 123,000 | |
| Alexa Fluor 680 | 679 | 702* | 184,000 | |
| Alexa Fluor 700 | 702 | 723* | 192,000 | |
| Alexa Fluor 750 | 749 | 775* | 240,000 | |
| Spectral characteristics of the Alexa Fluor dyes. Extinction coefficient at l max in cm -1m-1. *Human vision is insensitive to light beyond~650nm, and therefore it is not possible to view the far -red- fluorescent dyes by looking through the eyepiece of a conventional fluorescent microscope. Source: Molecular Probes www.probes.com |
| List of Other Molecular Probes Fluorescent Dyes | ||||
| Dye | Color | Absorbance max (nm) | Emission max (nm) | |
| Cascade Blue | Blue | 396 | 410 | |
| Marina Blue | Blue | 362 | 459 | |
| Oregon Green 500 | Green | 499 | 519 | |
| Oregon Green 514 | Green | 506 | 526 | |
| Oregon Green 488 | Green | 495 | 521 | |
| Oregon Green 488-X | Green | 494 | 517 | |
| Pacific Blue | Blue | 416 | 451 | |
| Rhodamine Green | Green | 504 | 532 | |
| Rhodol Green | Green | 496 | 523 | |
| Rhodamine Green-X | Green | 503 | 528 | |
| Rhodamine Red-X | Red | 560 | 580 | |
| Texas Red-X | Red | 583 | 603 | |
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*Disclaimer of License Statement for Molecular Beacons Products The 5' Nuclease detection assay and other homogeneous amplification methods used in connection with the Polymerase Chain Reaction ("PCR") Process are covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd. ("Roche"). Use of these methods requires a license. No license under these patents, which include but are not limited to United States Patent Nos. 5,210,015, 5,487,972, 5,804,375, and 5,994,076 to use the 5' Nuclease Assay or any Roche patented homogeneous amplification process is conveyed expressly or by implication to the purchaser by the purchase of any Gene Link, Inc. PCR-related product. Purchasers of these products must obtain a license to use the 5' Nuclease or Homogeneous PCR process before performing PCR. Further information on purchasing licenses to practice the PCR Process may be obtained by contacting the Licensing Specialist at (510) 814-2984, Roche Molecular Systems, Inc. 1145 Atlantic Avenue, Alameda, California, 94501 *PHRI Molecular Beacon License Agreement "This product is sold under license from the Public Health Research Institute. It may be used under PHRI Patent Rights only for the purchaser's research and development activities". **Black Hole Quencher License Agreement "Black Hole Quencher", "BHQ-1", "BHQ-2" and "BHQ-3" are registered trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ technology is licensed and sold under agreement with Biosearch and these products are sold exclusively for R&D use by the purchaser. They may not be used for clinical or diagnostic purposes and they may not be resold, distributed or re-packaged |
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