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Other Fluorophores for Molecular Probes

Applied Biosystems Proprietary Dyes & Possible Substitutions

Dye

Color

Absorbance max (nm)

Emission max (nm)

VIC Pink Red 538 554
HEX Pink Red 535 556
NED Red Orange 546 575
Cy3 Red Orange 550 570
PET Red Orange 558 595
Cy3.5 Red 588 604
ROX Red 575 602
Texas Red Red 583 603

Alexa Fluorescent Dye**

Alexa Fluor Dye

Color

Absorbance max (nm)

Emission max (nm)

Extinction Coefficient

Alexa Fluor 350   346 442 19,000
Alexa Fluor 405   401 421 34,000
Alexa Fluor 430   433 541 16,000
Alexa Fluor 488   495 519 71,000
Alexa Fluor 532   532 553 81,000
Alexa Fluor 546   556 573 104,000
Alexa Fluor 555   555 565 150,000
Alexa Fluor 568   578 603 91,000
Alexa Fluor 594   590 617 73,000
Alexa Fluor 633   632 647* 100,000
Alexa Fluor 647   650 665* 239,000
Alexa Fluor 660   663 690* 123,000
Alexa Fluor 680   679 702* 184,000
Alexa Fluor 700*   702 723* 192,000
Alexa Fluor 750*   749 775* 240,000

Spectral characteristics of the Alexa Fluor dyes. Extinction coefficient at l max in cm -1m-1. *Human vision is insensitive to light beyond~650nm, and therefore it is not possible to view the far -red- fluorescent dyes by looking through the eyepiece of a conventional fluorescent microscope. Source: Molecular Probes www.probes.com
** Alexa dye labelled product is provided under an intellectual property license from Thermo Fisher/Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The buyer cannot sell or otherwise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party or otherwise use this product or its components or materials made using this product or its components for Commercial Purposes. Commercial Purposes means any activity by a party for consideration and may include, but is not limited to: (1) use of the product or its components in manufacturing; (2) use of the product or its components to provide a service, information, or data; (3) use of the product or its components for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the product or its components, whether or not such product or its components are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

List of Other Molecular Probes Fluorescent Dyes

Dye

Color

Absorbance max (nm)

Emission max (nm)

Cascade Blue Blue 396 410
Marina Blue Blue 362 459
Oregon Green 500 Green 499 519
Oregon Green 514 Green 506 526
Oregon Green 488 Green 495 521
Oregon Green 488-X Green 494 517
Pacific Blue Blue 416 451
Rhodamine Green Green 504 532
Rhodol Green Green 496 523
Rhodamine Green-X Green 503 528
Rhodamine Red-X Red 560 580
Texas Red-X Red 583 603

**Black Hole Quencher License Agreement
"Black Hole Quencher", "BHQ-1", "BHQ-2" and "BHQ-3" are registered trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ technology is licensed and sold under agreement with Biosearch and these products are sold exclusively for R&D use by the purchaser. They may not be used for clinical or diagnostic purposes and they may not be resold, distributed or re-packaged

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