Quenchers - Introduction

Introduction to Quenchers

Quenchers Applications

Quenchers Design/Protocol

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Introduction to Quenchers

Quenchers are substances capable of absorbing energy from a fluorophore (such as a fluorescent dye) and re-emitting much of that energy as either heat (in the case of dark quenchers) or visible light (in the case of fluorescent quenchers). Dabcyl is an example of a dark quencher, and TAMRA is an example of a fluorescent quencher. When the fluorophore and quencher are in close proximity, the quencher absorbs the energy emitted from an excited fluorophore, thereby suppressing its emission. When the two substances are widely separated, the quencher no longer can absorb the fluorophore�s emission, and the latter�s presence can be visually detected (1). These properties are utilized in many popular oligonucleotide probes currently used for research or diagnostic purposes, such as TaqMan or Molecular Beacon probes (2-3). In such probes, a quencher and fluorophore having overlapping absorption and emission spectra, respectively, are incorporated into the probe as a pair. The probe is designed such that the quencher and fluorophore will always remain in close proximity if the specific target is not present, and be widely separated if it is present. Observation of a fluorescent signal thus indicates presence of target, and lack of a fluorescent signal indicates absence of target.

Quencher Spectral Data
Quencher	Absorption Max, nm	Quenching Range, nm
Dabcyl	453	380-530
BHQ-0	495	430-520
BHQ1	534	480-580
BHQ2	579	550-650
BHQ3	672	620-730
BBQ-650	650	550-750
**Black Hole Quencher License Agreement "Black Hole Quencher", "BHQ-1", "BHQ-2" and "BHQ-3" are registered trademarks of Biosearch Technologies, Inc., Novato, CA.

Dye & Quencher Spectral Overlap

Quenchers Modifications						View PDF	Excel Mods & Code List
Product	Code	Catalog No.	50 nmol scale (XX=05)	200 nmol scale (XX=02)	1 mmol scale (XX=01)	2 mmol scale (XX=03)	10 mmol scale (XX=10)	15 mmol scale (XX=15)
3'-BBQ-650 (Black Berry Quencher 650)	[3-BBQ-650]	26-6698	$97.00	$97.00	$162.00	$308.00	$1,296.00	$1,620.00
BHQ-0 (Black Hole Quencher 0, 3')	[BHQ-0-3]	26-6475	$76.00	$76.00	$98.00	$147.00	$786.00	$983.00
BHQ-1 (Black Hole Quencher 1, 3')	[3-BHQ-1]	26-6472	$76.00	$76.00	$98.00	$147.00	$786.00	$983.00
BHQ-2 (Black Hole Quencher 2, 3')	[3-BHQ-2]	26-6468	$76.00	$76.00	$98.00	$147.00	$786.00	$983.00
BHQ-3 (Black Hole Quencher 3, 3')	[3-BHQ-3]	26-6473	$97.00	$97.00	$320.00	$480.00	$2,561.00	$3,201.00
BHQ-1 (Black Hole Quencher-1, 5')	[5-BHQ-1]	26-6727	$427.00	$427.00	$481.00	$679.00	$3,624.00	$4,531.00
BHQ-2 (Black Hole Quencher-2, 5')	[5-BHQ-2]	26-6728	$240.00	$427.00	$481.00	$679.00	$3,624.00	$4,531.00
BHQ-3 (Black Hole Quencher-3, 5')	[5-BHQ-3]	26-6729	$322.50	$322.50	$419.50	$628.87	$3,356.00	$4,195.00
Atto 540Q	[Atto540Q-N]	26-6962	$702.00	$702.00	$913.00	$2,754.00	$7,301.00	$9,126.00
Atto 575Q	[Atto575Q-N]	26-6969	$702.00	$702.00	$913.00	$2,754.00	$7,301.00	$9,126.00
Atto 612Q	[Atto612Q-N]	26-6975	$702.00	$702.00	$913.00	$2,754.00	$7,301.00	$9,126.00
BBQ-650 NHS (Black Berry Quencher 650 NHS)	[BBQ-650 N]	26-6734	$227.00	$227.00	$416.00	$622.00	Inquire	Inquire
BBQ-650-dT (Black Berry Quencher 650 dT)	[BBQ-650-dT]	26-6699	$551.00	$551.00	$670.00	$848.00	$5,549.00	$6,936.00
BHQ-1-NHS (Black Hole Quencher 1 NHS)	[BHQ-1 N]	26-6732	$227.00	$227.00	$416.00	$622.00	Inquire	Inquire
BHQ-1-dT (Black Hole Quencher 1 dT)	[BHQ-1-dT]	26-6652	$482.00	$482.00	$578.00	$686.00	$3,313.00	$4,142.00
BHQ-2-dT (Black Hole Quencher 2 dT)	[BHQ-2-dT]	26-6653	$482.00	$482.00	$578.00	$686.00	$3,313.00	$4,142.00
Dabcyl Quencher deoxythymidine dT	[Dab-dT]	26-6446	$459.00	$459.00	$632.00	$684.00	$3,650.00	$4,563.00
Dabcyl Quencher-3'	[Dab-3]	26-6470	$159.00	$159.00	$207.00	$305.00	$1,160.00	$1,315.00
Dabcyl-5'	[Dab-5]	26-6704	$267.00	$267.00	$347.00	$521.00	$3,475.00	$2,780.00
BBQ-650 Internal	[BBQ-650-Int]	26-6776	$583.00	$583.00	$659.00	$821.00	$5,270.00	$6,588.00
BHQ-1 Internal	[BHQ-1-Int]	26-6775	$583.00	$583.00	$659.00	$821.00	$5,270.00	$6,588.00
BHQ-2 Internal	[BHQ-2-Int]	26-6772	$583.00	$583.00	$659.00	$821.00	$5,270.00	$6,588.00
MGB 3' CDPI3	[MGB-CDPI3-3]	26-6456	$348.00	$348.00	$443.00	$589.00	$1,782.00	$2,145.00
MGB-5' CDPI3	[MGB-CDPI3-5]	26-6457	$880.00	$880.00	$1,782.00	$1,901.00	$8,532.00	$10,260.00
Tamra NHS	[Tamra-N]	26-6450	$378.00	$378.00	$491.00	$413.00	$3,931.00	$4,914.00
TAMRA-3' (Carboxytetramethylrhodamine)	[3-Tamra]	26-6451	$130.00	$130.00	$205.00	$308.00	$2,462.00	$3,078.00
Tamra-dT (Carboxytetramethylrhodamine-dT)	[Tamra-dT]	26-6449	$378.00	$378.00	$486.00	$729.00	$3,888.00	$4,860.00

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Quenchers - Introduction

Introduction to Quenchers

Quenchers Modifications