Quick Order |All Online Ordering|Product Catalog Ordering|Oligo Modifications List|Product Info & Literature|Oligo Design Tools/Resources

Dabcyl-5

Modification : Dabcyl-5

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6704
Quenchers
Dab
Y
Y
N
462.44
11.1
PS26-6704.pdf
-
-
-

picture of Dabcyl-5


Catalog NoScalePrice
26-6704-0550 nmol$247.50
26-6704-02200 nmol$247.50
26-6704-011 umol$321.75
26-6704-032 umol$482.62
26-6704-1010 umol$3,217.50
26-6704-1515 umol$2,574.00
Discounts are available for Dabcyl-5!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Dabcyl-5 is classified as a dark quencher (a non-fluorescent chromophore), and is typically used to label a Fluorescence Resonance Energy Transfer (FRET) DNA oligonucleotide probe with a quencher moiety at the 5-end. TaqMan or Molecular Beacon probes labeled with Dabcyl at the 5-end usually would be labeled with an appropriate fluorescent dye at the 3-end; other kinds of FRET probes might have the fluorescent dye at an internal position. 5-end labeling such probes with Dabcyl is unusual. The primary reason for using this inverse-labeling strategy is to take advantage of Dabcyls very high hydrophobicity (which is higher than that of a fluorescent dye) to improve subsequent purification of the probe by HPLC or RPC. For example, a molecular beacon with 5-Dabcyl and 3-6-FAM is easier to purify (and will subsequently be purer) than a probe with the more common, opposite set-up.


Quencher Spectral Data

Quencher

Absorption Max, nm

Quenching Range, nm

Dabcyl 453 380-530
BHQ1 534 480-580
BHQ2 579 550-650
BHQ3 672 620-730
BBQ-650 650 550-750
Click here for complete list of quenchers
**Black Hole Quencher License Agreement
"Black Hole Quencher", "BHQ-1", "BHQ-2" and "BHQ-3" are registered trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ technology is licensed and sold under agreement with Biosearch and these products are sold exclusively for R&D use by the purchaser. They may not be used for clinical or diagnostic purposes and they may not be resold, distributed or re-packaged.

- Dabcyl-5

Oligonucleotide Synthesis |  Flourescent Molecular Probes |  Gene Detection Systems |  Tools & Reagents |  Gene Assays |  RNAi
© 2017 Gene Link |  Terms & Conditions |  Licenses |  Privacy Policy |  May 28, 2017 5:01:05 PM