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Conjugation or Surface Attachment - Introduction

Introduction to Conjugation or Surface Attachment Conjugation or Surface Attachment Applications Conjugation or Surface Attachment Design/Protocol Conjugation or Surface Attachment Literature Order Online

Introduction to Conjugation or Surface Attachment

Incorporation of reactive organic functional groups, particularly primary amine, thiol (sulfhydryl), or carboxylate groups, at specific sites within an oligonucleotide allows for subsequent conjugation of the oligo with a number of different affinity, reporter or protein labels, depending on the application. Such labels need to be appropriately reactive to the incorporated functional group, for example, NHS esters or isothiocyanates in the case of primary amines, and iodoacetamides or maleimides in the case of primary thiols. Examples of labels includes fluorescent dyes/quenchers, digoxigenin, biotin, and enzymes. Functionally-derivitized oligos can also be covalently attached to surfaces such as glass slides or gold microspheres for use in various microarray or nanoelectronic applications.


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The primary amine labelled oligos can also be conjugated to carboxyl functional groups usually for solid supports applications using EDC mediated reaction as shown in the figure below.





Conjugation or Surface Attachment Modifications
Product Code Catalog No. 50 nmol scale (XX=05) 200 nmol scale (XX=02) 1 mmol scale (XX=01) 2 mmol scale (XX=03) 10 mmol scale (XX=10) 15 mmol scale (XX=15)

Alkyne-Modifier Serinol

[Alkyne Ser]

26-6925

$240.50

$240.50

$312.65

$468.97

$2,501.20

$3,126.50

Alkyne- C2 NHS

[Alkyne-C2 N]

26-6924

$185.00

$185.00

$275.00

$315.00

$936.00

$1,170.00

Amino C6 U (RNA)

[Amino C6 U]

27-6422

$220.50

$220.50

$286.65

$429.97

$2,293.20

$2,866.50

Amino deoxyadenosine dA C6

[Am dA C6]

26-6666

$225.50

$225.50

$293.15

$438.75

$2,345.20

$2,931.50

Amino doxycytosine dC C6

[Am dC C6]

26-6670

$247.50

$247.50

$321.75

$482.62

$2,574.00

$3,217.50

Amino deoxyguanosine dG C6

[Am dG C6]

26-6669

$480.00

$480.00

$624.00

$936.00

$4,992.00

$6,240.00

Amino deoxythymidine dT C6

[Am dT C6]

26-6438

$184.00

$184.00

$280.80

$421.20

$2,246.40

$2,808.00

Amino Linker C12

[AmC12]

26-6420

$117.00

$117.00

$152.10

$228.15

$1,216.80

$1,521.00

Amino Linker C3

[AmC3]

26-6405

$45.00

$45.00

$58.50

$87.75

$468.00

$585.00

Amino Linker C6

[AmC6]

26-6418

$24.00

$24.00

$58.50

$66.80

$249.60

$312.00

Amino Linker C7

[AmC7]

26-6406

$45.00

$45.00

$58.50

$87.75

$468.00

$585.00

Amino modifier serinol

[AmSr]

26-6715

$247.50

$247.50

$321.75

$482.62

$2,574.00

$3,217.50

Azide butyrate NHS

[N3-C4 N]

26-6922

$120.00

$120.00

$156.00

$234.00

$1,248.00

$1,560.00

5-Carboxy-C10

[Ca-C10]

26-6717

$64.00

$64.00

$96.00

$144.00

$720.00

$936.00

Carboxy-deoxythymidine

[CO-dT]

26-6697

$216.00

$216.00

$280.80

$421.20

$2,246.40

$2,808.00

Maleimide-Modifier (5')

[Mal]

26-6574

$105.00

$105.00

$136.50

$204.75

$816.00

$1,368.00

PC Amino C6 (Photocleavable)

[PCAmC6]

26-6690

$170.00

$170.00

$210.00

$330.00

$1,680.00

$2,100.00

6-Thio-dG (S6-dG)

[S6-dG]

26-6533

$410.00

$410.00

$533.40

$789.50

$4,267.20

$5,334.00

4-Thio dU (s4dU)

[s4dU]

26-6445

$354.00

$354.00

$460.20

$531.00

$3,681.60

$4,602.00

4-Thio-dT (S4dT)

[S4dT]

26-6538

$354.00

$354.00

$460.20

$531.00

$3,681.60

$4,602.00

4-Thio-Uridine (s4U)

[s4U]

27-6445

$310.00

$310.00

$558.00

$669.60

$3,348.00

$5,580.00

Thiol C6 dT

[S-C6-dT]

26-6483

$185.00

$185.00

$370.00

$457.00

$2,220.00

$3,700.00

Thiol SS Serinol Dipod (DTSPA)

[DTSPA]

26-6573

$287.00

$287.00

$373.10

$430.50

$2,984.80

$3,731.00

Thiol SS-C3

[SS-C3]

26-6482

$210.00

$210.00

$290.00

$395.00

$2,127.00

$2,450.00

Thiol SS-C6

[SS-C6]

26-6419

$98.00

$98.00

$127.40

$191.10

$1,019.00

$1,274.00


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