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Duplex Stability - Introduction

Introduction to Duplex Stability Duplex Stability Applications Duplex Stability Design/Protocol Duplex Stability Literature Order Online

Introduction to Duplex Stability

Many molecular biology applications require oligonucleotide primers and/or probes with enhanced duplex stability, that is, a higher affinity for their complementary sequences. Examples include antisense, siRNA, and SNP detection experiments. For these and other applications, enhancing primer/probe-target duplex stability increases both the selectivity and sensitivity of the oligo for its target. An oligos binding affinity for its target can be manipulated by incorporating various modifications into it, either on the ends or at an internal position (1). Examples include 2-OMethyl base, 2-Amino-dA, 5-methyl-dC and 2-Fluoro bases. Changes in duplex stability incurring from such modifications is indicated by changes in the melting temperature (Tm) of the duplex formed between the modified oligo and its target over that formed by the unmodified oligo. Depending on the modification, Tm can be increased over a range of 0.5C to 3.0C per base substitution.

Duplex Stability Modifications
Product Catalog No. 50 nmol scale (XX=05) 200 nmol scale (XX=02) 1 mmol scale (XX=01) 2 mmol scale (XX=03) 10 mmol scale (XX=10) 15 mmol scale (XX=15)

2'-Fluoro deoxyadenosine (2'-F-A)

26-6692

$38.00

$38.00

$57.00

$85.50

$456.00

$570.00

2'-Fluoro deoxycytosine (2'-F-C)

26-6463

$28.00

$28.00

$36.40

$54.60

$291.20

$364.00

2'-Fluoro deoxyguanosine (2'-F-G)

26-6693

$38.00

$38.00

$57.00

$85.50

$456.00

$570.00

2'-Fluoro deoxyuridine (2'-F-U)

26-6462

$28.00

$28.00

$36.40

$54.60

$291.20

$364.00

2'-O methyl adenosine A

27-6410A

$6.00

$8.00

$16.00

$28.00

$102.00

$148.00

2'-O methyl cytosine C

27-6410C

$6.00

$8.00

$16.00

$28.00

$102.00

$148.00

2'-O methyl guanosine G

27-6410G

$6.00

$8.00

$16.00

$28.00

$102.00

$148.00

2'-O methyl uridine U

27-6410U

$6.00

$8.00

$16.00

$28.00

$102.00

$148.00

2-Amino dA

26-6525

$162.50

$162.50

$211.25

$316.87

$1,690.00

$2,112.50

5-carboxy dC

26-6891

$550.00

$550.00

$715.00

$1,072.50

$5,720.00

$7,150.00

5-methyl deoxycytosine (5Me-dC)

26-6413

$49.00

$49.00

$63.70

$95.55

$509.60

$637.00

AP-dC G-Clamp (Aminoethyl-Phenoxazine-dC (AP-dC))

26-6920

$460.00

$460.00

$897.00

$897.00

$4,784.00

$5,980.00

d5SICS

26-6562

$1,285.20

$1,285.20

$1,670.76

$2,506.14

$13,366.08

$16,707.60

dDs (7-(2-thienyl)-imidazo[4,5-b]pyridine)

26-6565

$188.50

$188.50

$245.05

$367.57

$1,960.40

$2,450.50

dDss

26-6566

$300.00

$300.00

$390.00

$585.00

$3,120.00

$3,900.00

dNaM

26-6561

$622.50

$622.50

$809.25

$1,213.87

$6,474.00

$8,092.50

dPa pyrrole-2-carbaldehyde (Pa)

26-6564

$169.00

$169.00

$219.70

$329.55

$1,757.60

$2,197.00

N4-Ethyl dC

26-6685

$350.00

$350.00

$425.00

$637.50

$3,400.00

$4,250.00

N6-Methyl dA (m6dA)

26-6601

$162.50

$162.50

$211.25

$316.87

$1,690.00

$2,112.50

propyne dC

26-6501

$130.00

$130.00

$200.00

$265.00

$1,600.00

$2,000.00

propyne dU

26-6502

$97.50

$97.50

$126.75

$190.12

$1,014.00

$1,267.50

Pyrene Cap (5')

26-6949

$253.50

$253.50

$329.55

$494.32

$2,636.40

$3,295.50

Spermine Oligo Cationic Tail

26-6454

$324.00

$324.00

$421.20

$631.80

$3,369.60

$4,212.00

2-Thio-dT (S2dT)

26-6537

$354.00

$354.00

$460.20

$690.30

$3,681.60

$4,602.00

Stilbene Cap: Trimethoxystilbene Cap (5')

26-6989

$253.50

$253.50

$329.55

$494.32

$2,636.40

$3,295.50

Uaq Cap (3')

26-6950

$234.00

$234.00

$304.20

$456.30

$2,433.60

$3,042.00


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