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Nuclease Resistance - Introduction

Introduction to Nuclease Resistance Nuclease Resistance Applications Nuclease Resistance Design/Protocol Nuclease Resistance Literature Order Online

Introduction to Nuclease Resistance

Like cellular DNA and RNA, synthetic oligonucleotides are prone to degradation once introduced into the cell or body fluids. Such degradation is due to the ubiquitous presence of nuclease enzymes (both exonucleases and endonucleases), as well as chemical instability (particularly for RNA). Under normal cellular conditions, this leads to fast in vivo degradation of oligos and a short half-life (1). In addition, the ease with which RNAse, being very stable, can contaminate laboratory equipment and benchtop surfaces means that RNA is susceptible to degradation under normal laboratory conditions as well. To reduce or eliminate this susceptibility, nuclease-resistant modifications (for example, phosphorothiolation or 2-OMethyl RNA bases) can be introduced into oligonucleotides slated for in vivo and/or regular benchtop work.

Nuclease Resistance Modifications
Product Catalog No. 50 nmol scale (XX=05) 200 nmol scale (XX=02) 1 mmol scale (XX=01) 2 mmol scale (XX=03) 10 mmol scale (XX=10) 15 mmol scale (XX=15)

2'-Fluoro deoxyadenosine (2'-F-A)

26-6692

$38.00

$38.00

$57.00

$85.50

$456.00

$570.00

2'-Fluoro deoxycytosine (2'-F-C)

26-6463

$28.00

$28.00

$36.40

$54.60

$291.20

$364.00

2'-Fluoro deoxyguanosine (2'-F-G)

26-6693

$38.00

$38.00

$57.00

$85.50

$456.00

$570.00

2'-Fluoro deoxyuridine (2'-F-U)

26-6462

$28.00

$28.00

$36.40

$54.60

$291.20

$364.00

2'-O methyl adenosine A

27-6410A

$6.00

$8.00

$16.00

$28.00

$102.00

$148.00

2'-O methyl cytosine C

27-6410C

$6.00

$8.00

$16.00

$28.00

$102.00

$148.00

2'-O methyl guanosine G

27-6410G

$6.00

$8.00

$16.00

$28.00

$102.00

$148.00

2'-O methyl uridine U

27-6410U

$6.00

$8.00

$16.00

$28.00

$102.00

$148.00

L-DNA dA

26-6941

$187.00

$187.00

$336.60

$504.90

$1,992.80

$2,266.00

L-DNA dC

26-6942

$187.00

$187.00

$336.60

$504.90

$1,992.80

$2,266.00

L-DNA dG

26-6943

$187.00

$187.00

$336.60

$504.90

$1,992.80

$2,266.00

L-DNA dT

26-6944

$187.00

$187.00

$336.60

$504.90

$1,992.80

$2,266.00

L-RNA rA

27-6941

$187.00

$187.00

$336.60

$504.90

$1,992.80

$2,266.00

L-RNA rC

27-6942

$187.00

$187.00

$336.60

$504.90

$1,992.80

$2,266.00

L-RNA rG

27-6943

$187.00

$187.00

$336.60

$504.90

$1,992.80

$2,266.00

L-RNA rU

27-6944

$187.00

$187.00

$336.60

$504.90

$1,992.80

$2,266.00

phosphorothioate

26-6401

$3.50

$4.25

$6.50

$9.00

$52.00

$65.00

propyne dC

26-6501

$130.00

$130.00

$200.00

$265.00

$1,600.00

$2,000.00

propyne dU

26-6502

$97.50

$97.50

$126.75

$190.12

$1,014.00

$1,267.50


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