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Cell Membrane Permeation - Introduction

Introduction to Cell Membrane Permeation Cell Membrane Permeation Applications Cell Membrane Permeation Design/Protocol Cell Membrane Permeation Literature Order Online

Introduction to Cell Membrane Permeation

Oligonucleotides are single-stranded nucleic acid molecules, and due to their phosphodiester backbones, are predominantly hydrophilic. While this is usually not a problem for in vitro applications, for in vivo work, the hydrophobic nature of the cell membrane makes it difficult for oligos to permeate it and enter the cell. The need to modify oligos to improve their membrane permeability and cellular uptake can be critical in anti-sense, siRNA, or in vivo diagnostic applications. Generally speaking, such modifications need to be both hydrophobic and relatively non-toxic. Modification of oligos at the 5- or 3-ends with cholesterol or PEG are strategies commonly used to achieve this.

Cell Membrane Permeation Modifications
Product Code Catalog No. 50 nmol scale (XX=05) 200 nmol scale (XX=02) 1 mmol scale (XX=01) 2 mmol scale (XX=03) 10 mmol scale (XX=10) 15 mmol scale (XX=15)

Cholesterol TEG-5' (15 atom triethylene glycol spacer)









Spacer 18 (hexaethyleneglycol)









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