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In this section: Introduction | Genemerâ„¢ | GeneProberâ„¢ | Proberâ„¢ Gene Detection Kits | GScanâ„¢ Gene Detection Kits | Genemerâ„¢ Control DNA | Infectious Diseases
In this section: Gene Construction
In this section: Introduction | The Omni-Cleanâ„¢ System | The Omni-Pureâ„¢ Plasmid Purification System | The Omni-Pureâ„¢ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
In this section: Introduction | Quality Control | Purification | Modifications | Long Oligos | Price List
In this section: Introduction | Molecular Beacon FAQ's | Fluorescent Probes Price List | Other Fluorescent Molecular Probes
In this section: SPCT | DME SPCT Intro | Order DME TaqMan® Assays SPCT | SNP PCT Search | Gene Expression Assays | SPCT Design Center | GeneAssays
In this section: RNA Oligonucleotides | Quality Control | Purification | Modifications | RNAi Explorerâ„¢ Products and Prices | Custom RNAi | RNAi Design Guidelines | SmartBaseâ„¢ siRNA Modifications | shRNA Explorerâ„¢
In this section: PCR Amplification & Analysis
In this section: Introduction | Genemerâ„¢ | GeneProberâ„¢ | Proberâ„¢ Gene Detection Kits | GScanâ„¢ Gene Detection Kits | Genemerâ„¢ Control DNA | Infectious Diseases
In this section: Gene Construction
In this section: Introduction | The Omni-Cleanâ„¢ System | The Omni-Pureâ„¢ Plasmid Purification System | The Omni-Pureâ„¢ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
Puromycin
Modification : Puromycin
Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC
Catalog No | Scale | Price | 26-6603-05 | 50 nmol | $76.00 | 26-6603-02 | 200 nmol | $76.00 | 26-6603-01 | 1 umol | $98.80 | 26-6603-03 | 2 umol | $148.20 | 26-6603-10 | 10 umol | $790.40 | 26-6603-15 | 15 umol | $988.00 |
Puromycin can be attached to the 3’ end of RNA and DNA oligos. Puromycin is an antibiotic that mimics transfer RNA. Puromycin binds in the ribosome's A site and forms a peptide bond with the growing peptide chain to block peptide elongation. By linking puromycin to synthetic RNA; a peptide-RNA fusion product can be formed.
An application example is the use of 3'Puromycin to synthesize d(A27CC)-puromycin to which various mRNA sequences were then ligated. The mRNA sequence information was then translated in a reticulocyte lysate system. As the ribosome reached the poly-dA sequence, translation was stalled. Puromycin entered the ribosome A site and a peptide bond formed between the C-terminal of the synthesized peptide and the RNA encoding the peptide structure. The poly-dA sequence serves two purposes, first it stalls the ribosome thereby allowing puromycin to enter the A site and second it acts as a future capture site for oligo-dT-biotin.
References:
(1) S. Borman, C&EN, Feb. 12, 1996, 29-54.
(2) R.W. Roberts and J.W. Szostak, Proc. Natl. Acad. Sci. USA, 1997, 94, 12297-302
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