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Puromycin

picture of Puromycin

Modification : Puromycin

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6603
Others
Puro
N
Y
N
533.48
19.5
PS26-6603.pdf
-
-
-


Catalog NoScalePrice
26-6603-0550 nmol$185.00
26-6603-02200 nmol$185.00
26-6603-011 umol$210.00
26-6603-032 umol$395.00
26-6603-1010 umol$1,680.00
26-6603-1515 umol$2,100.00

Puromycin can be attached to the 3 end of RNA and DNA oligos. Puromycin is an antibiotic that mimics transfer RNA. Puromycin binds in the ribosome's A site and forms a peptide bond with the growing peptide chain to block peptide elongation. By linking puromycin to synthetic RNA; a peptide-RNA fusion product can be formed. An application example is the use of 3'Puromycin to synthesize d(A27CC)-puromycin to which various mRNA sequences were then ligated. The mRNA sequence information was then translated in a reticulocyte lysate system. As the ribosome reached the poly-dA sequence, translation was stalled. Puromycin entered the ribosome A site and a peptide bond formed between the C-terminal of the synthesized peptide and the RNA encoding the peptide structure. The poly-dA sequence serves two purposes, first it stalls the ribosome thereby allowing puromycin to enter the A site and second it acts as a future capture site for oligo-dT-biotin. References: (1) S. Borman, C&EN, Feb. 12, 1996, 29-54. (2) R.W. Roberts and J.W. Szostak, Proc. Natl. Acad. Sci. USA, 1997, 94, 12297-302 - Puromycin

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