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2'-O methyl adenosine A

picture of 2'-O methyl adenosine A

Modification : 2'-O methyl A

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



27-6410A
Nuclease Resistance
mA
Y
Y
Y
343.24
15.4
PS27-6410.pdf
-
-
-


Catalog NoScalePrice
27-6410A-0550 nmol$6.00
27-6410A-02200 nmol$8.00
27-6410A-011 umol$16.00
27-6410A-032 umol$28.00
27-6410A-1010 umol$102.00
27-6410A-1515 umol$148.00
Discounts are available for 2'-O methyl A!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

2-O-Methyl adenosine A is classified as a 2-O-Methyl RNA monomer. 2-O-Methyl nucleotides are most commonly used to confer nuclease resistance to an oligo designed for anti-sense, siRNA or aptamer-based research, diagnostic or therapeutic purposes, when specific 2-OH is not required. Nuclease resistance can be further enhanced by phosphorothiolation of appropriate internucleotide linkages within the oligo.

The hydrogen bonding behavior of a 2-O-Methyl RNA/RNA base pair is closer to that of an RNA/RNA base pair than a DNA/RNA base pair. Consequently, the presence of 2-O-Methyl nucleotides improves duplex stability. Indeed, incorporation of a 2-O-Methyl nucleotide into an anti-sense oligo (resulting in a 2-O-Methyl RNA/DNA chimeric), lead to a increase in the Tm of its duplex with RNA, relative to that formed by an unmodified anti-sense DNA oligo, of 1.3C per 2-O-Methyl RNA residue added (2). Moreover, from a synthesis standpoint, the coupling efficiency of 2-O-Methyl phosphoramidites are higher than those of RNA monomers, resulting in higher yield of full-length oligos.

References
1. Cotton, M.; Oberhauser, B.; Burnar, H. et al. 2O methyl and 2O ethyl oligoribonucleotides as inhibitors of the in vitro U7 snRNP-dependent messenger-RNA processing event. Nucleic Acids Res. (1991) , 19:2629-2635.
2. Kawasaki, A.M. et al., Uniformly modified 2'-deoxy-2'-fluoro phosphorothioate oligonucleotides as nuclease resistant antisense compounds with high affinity and specificity for RNA targets, Journal of Medicinal Chemistry (1993), 36: 831-841.
- 2'-O methyl adenosine A

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