Amino Allyl

Code : [AmAllyl]

Modification : Amino Allyl

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6731
Conjugation Chemistry
[AmAllyl]
Y
Y
Y
101.14
-
26-6731.pdf
-
-
-

Catalog No	Scale	Price
26-6731-05	50 nmol	$227.00
26-6731-02	200 nmol	$227.00
26-6731-01	1 umol	$276.00
26-6731-03	2 umol	$335.00
26-6731-10	10 umol	$1,674.00
26-6731-15	15 umol	$1,895.00

Discounts are available for Amino Allyl!
Modification* Discount Price Structure
1 site/order	List price
2 sites/order	10% discount
3 sites/order	20% discount
4 sites/order	30% discount
5-9 sites/order	50% discount
10+ sites/order	60% discount
*Exceptions apply

Related Modifications
Amino C6	Amino dA C6	Amino Serinol
Amino Allyl rU	Amino dT C6	Amino Allyl dU
Amino dG C6	Amino C3	Amino C12
Amino C6 U	Amino Spacer 7 C6 Internal	Amino C7- 3'
Amino dC C6

Amino Allyl can be used to incorporate an active primary amino group onto the 5'-end of an oligonucleotide. This can then be conjugated to a NHS Activated ligand. The amino group then becomes internal to the 5' end ligand. The amino group is separated from the 5'-end nucleotide base by a 4-carbon spacer arm to reduce steric interaction between the amino group and the oligo.
The presence of the primary amino group allows the user to label the oligo with a variety of different ligands for affinity, reporter or protein moieties (as NHS esters or isothiocyanates), depending on the application. Examples include biotin, digoxigenin, and fluorescent dyes or quenchers, magnetic beads and enzymes (for example, alkaline phosphatase).

The primary amine labelled oligos can also be conjugated to carboxyl functional groups usually for solid supports applications using EDC mediated reaction as shown in the figure below.

- Amino Allyl