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Tamra-dT (Carboxytetramethylrhodamine-dT)

Modification : Tamra-dT

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6449
Fluorescent Dyes
Tm-dT
Y
Y
Y
870.85
40.38
PS26-6449.pdf
565
580
-

picture of Tamra-dT (Carboxytetramethylrhodamine-dT)


Catalog NoScalePrice
26-6449-0550 nmol$350.00
26-6449-02200 nmol$350.00
26-6449-011 umol$450.00
26-6449-032 umol$675.00
26-6449-1010 umol$3,600.00
26-6449-1515 umol$4,500.00
Related Modifications
Tamra-3'
Tamra-5'
Tamra-dT
Tamra NHS

Carboxytetramethylrhodamine-deoxythymidien (TAMRA-dT) is a deoxythymidine nucleoside derivitized with TAMRA through a spacer arm. TAMRA-dT is used to internally label an oligonucleotide at a dT position. TAMRA-dT has an absorbance maximum of 565 nm and an emission maximum of 580 nm. TAMRA-dT can be used to internally label a Fluorescence Resonance Energy Transfer (FRET) DNA oligonucleotide probe with a quencher moiety. Such a labeling strategy is pertinent in cases where the distance between the quencher and fluorophore needs optimization for efficient quenching. For such probes, 6-FAM is most commonly used as the reporter moiety as the two dyes have excellent spectral overlap.

TAMRA-dT also can be used to label DNA oligos for use as hybridization probes in a variety of in vivo and in vitro research or diagnostic applications, as well as for structure-function studies of DNA, RNA, and protein-oligonucleotide complexes. Oligos internally labeled with TAMRA-dT also can be used as PCR and DNA sequencing primers to generate fluorescently-labeled PCR, sequencing or genetic analysis (AFLP or microsatellite) products. For further details concerning the TAMRA dye, please see the technical sheet for it.

- Tamra-dT (Carboxytetramethylrhodamine-dT)

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