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Fluorescein deoxythymidine dT

picture of Fluorescein deoxythymidine dT

Modification : Fluorescein dT

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6422
Fluorescent Dyes
FluorT
Y
Y
Y
815.71
38.8
PS26-6422.pdf
492
517
-


Catalog NoScalePrice
26-6422-0550 nmol$295.00
26-6422-02200 nmol$295.00
26-6422-011 umol$390.00
26-6422-032 umol$450.00
26-6422-1010 umol$3,120.00
26-6422-1515 umol$4,500.00
Discounts are available for Fluorescein dT!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Fluorescein-dT is a deoxythymidine nucleoside derivitized with 6-FAM (6-carboxyfluorescein) through a spacer arm. 6-FAM is the most commonly used fluorescent dye for labeling oligonucleotides; Fluorescein-dT is used to internally label an oligonucleotide at a dT position. Fluorescein-dT has an absorbance maximum of 492 nm and an emission maximum of 517 nm. Fluorescein-dT can be used to internally label a Fluorescence Resonance Energy Transfer (FRET) DNA oligonucleotide probe with a fluorophore. Such a labeling strategy is pertinent in cases where the distance between the quencher and fluorophore needs optimization for efficient quenching. For such probes, fluorescein is most commonly paired with the dark quencher BHQ-1, as the two have excellent spectral overlap.

Fluorescein-dT also can be used to label DNA oligos for use as hybridization probes in a variety of in vivo and in vitro research or diagnostic applications, as well as for structure-function studies of DNA, RNA, and protein-oligonucleotide complexes. Oligos internally labeled with fluorescein-dT also can be used as PCR and DNA sequencing primers to generate fluorescently-labeled PCR, sequencing or genetic analysis (AFLP or microsatellite) products.

- Fluorescein deoxythymidine dT

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