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Cy5.5 (Cyanine 5.5)

picture of Cy5.5 (Cyanine 5.5)

Modification : Cy5.5

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6460
Fluorescent Dyes
Cy5.5
Y
Y
Y
633.74
21.5
PS26-6460.pdf
675
694
-


Catalog NoScalePrice
26-6460-0550 nmol$450.00
26-6460-02200 nmol$450.00
26-6460-011 umol$585.00
26-6460-032 umol$877.50
26-6460-1010 umol$4,680.00
26-6460-1515 umol$5,850.00
Discounts are available for Cy5.5!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Cyanine 5.5 (Cy5.5) is a fluorescent dye that belongs to the Cyanine family of synthetic polymethine dyes. Cy5.5 is reactive, water-soluble, and has an absorbance maximum of 675 nm and an emission maximum of 694 nm. It is available as a phosphoramidite, and is used to fluorescently label oligonucleotides at either the 5- or 3-end, or internally. Cy5.5 plays a particularly important role in real-time PCR applications, being used as a reporter moiety in TaqMan probes (1), Scorpion primers (2) and Molecular Beacons (3). For such probes, Cy5.5 is most commonly paired with the dark quencher BHQ-3, as the two have excellent spectral overlap.

Cy5.5 can also be used to label DNA oligos for use as hybridization probes in other applications, such as Fluorescent In-Situ Hybridization (FISH).

References
1. Livak, K.J., Flood, S.J.A., Marmaro, J., Giusti, W., Deetz, K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.PCR Methods Appl. (1995), 4: 1-6.
2. Thelwell, N., Millington, S., Solinas, A., Booth, J., Brown, T. Mode of action and application of Scorpion primers to mutation detection. Nucleic Acids Res. (2000), 28: 3752-3761.
3. Tyagi, S., Kramer, F.R. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. (1996), 14: 303-308.
- Cy5.5 (Cyanine 5.5)

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