Modification : Cy5.5
Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC
26-6460
Fluorescent Dyes
[Cy5.5]
Y
Y
Y
633.74
21.5
PS26-6460.pdf
675
694
-
Catalog No | Scale | Price |
26-6460-05 | 50 nmol | $450.00 |
26-6460-02 | 200 nmol | $450.00 |
26-6460-01 | 1 umol | $585.00 |
26-6460-03 | 2 umol | $877.50 |
26-6460-10 | 10 umol | $4,680.00 |
26-6460-15 | 15 umol | $5,850.00 |
Discounts are available for Cy5.5! |
Modification* Discount Price Structure |
1 site/order
|
List price
|
2 sites/order
|
10% discount
|
3 sites/order
|
20% discount
|
4 sites/order
|
30% discount
|
5-9 sites/order
|
50% discount
|
10+ sites/order
|
60% discount
|
*Exceptions apply
|
Cyanine 5.5 (Cy5.5) is a far-red/near infra red (NIR) fluorescent dye that belongs to the Cyanine family of synthetic polymethine dyes. Cy5.5 is reactive, water-soluble, and has an absorbance maximum of 675 nm and an emission maximum of 694 nm. It is available as a phosphoramidite, and is used to fluorescently label oligonucleotides at either the 5' or 3' end, or internally. Cy5.5 plays a particularly important role in real-time PCR applications, being used as a reporter moiety in TaqMan probes (1), Scorpion primers (2) and Molecular Beacons (3). For such probes, Cy5.5 is most commonly paired with the dark quencher BHQ-3, as the two have excellent spectral overlap.
Cy5.5 can also be used to label DNA oligos for use as hybridization probes in other applications, such as Fluorescent In-Situ Hybridization (FISH).
References
1. Livak, K.J., Flood, S.J.A., Marmaro, J., Giusti, W., Deetz, K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.
PCR Methods Appl. (1995),
4: 1-6.
2. Thelwell, N., Millington, S., Solinas, A., Booth, J., Brown, T. Mode of action and application of Scorpion primers to mutation detection.
Nucleic Acids Res. (2000),
28: 3752-3761.
3. Tyagi, S., Kramer, F.R. Molecular beacons: probes that fluoresce upon hybridization.
Nat. Biotechnol. (1996),
14: 303-308.
- Cy5.5 (Cyanine 5.5)