Quick Order |All Online Ordering|Product Catalog Ordering|Oligo Modifications List|Product Info & Literature|Oligo Design Tools/Resources

Cy7 (Cyanine 7)

picture of Cy7 (Cyanine 7)

Modification : Cy7 NHS Ester

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6474
Fluorescent Dyes
Cy7-NHS
Y
Y
Y
682
12.5
PS26-6474.pdf
747
776
-


Catalog NoScalePrice
26-6474-0550 nmol$325.20
26-6474-02200 nmol$325.20
26-6474-011 umol$422.76
26-6474-032 umol$634.14
26-6474-1010 umol$3,382.08
26-6474-1515 umol$4,227.60

Cy7 modification is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is required. A C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

Cyanine 7(Cy7) NHS ester is a fluorescent dye that belongs to the Cyanine family of synthetic polymethine dyes. Cy7 is reactive, water-soluble, and has an absorbance maximum of 747 nm and an emission maximum of 776 nm, which is in the near IR. It is available as an NHS ester, and is used to fluorescently label oligonucleotides at either the 5- or 3-end, or internally. Because it is a near IR dye, Cy7 has very little background fluorescence associated with it (1). It is thus an excellent choice for labeling oligo probes slated for in vivo applications, because the minimal scattering and absorption of near-IR photons by cellular tissue ensures higher S/N ratio, and better sensitivity. For example, Fluorescent Resonance Energy Transfer (FRET) oligonucleotide duplexes using Cy5.5 as the donor on one strand and Cy7 as the acceptor on the complementary strand have been used to detect and characterize transcription factor NF-kappaB p50 protein binding to DNA (2)

Caution: Cy7 is intensely colored and very reactive. Care should be exercised when handling the vial containg the C7-labeled oligo to avoid staining clothing, skin, and other items. Also, because Cy7 is in the form of an NHS ester, the oligo first must be synthesized with an Amino C6 Linker (for the ends) or the Amino C6 version of the base phosphoramidite (for internal labeling). The Cy7-NHS ester is then manually attached to the oligo through the amino group in a separate reaction post-synthesis.

References
1. Benson, R.C., Kues, H.A. Absorption and Fluorescence Properties of Cyanine Dyes.J. Chem. Eng. Data (1977), 22: 379-383.
2. Zhang, S., Metelev, V., Tabatadze, D., Zamecnik, P.C., Bogdanov, A. Fluorescence resonance energy transfer in near-infrared fluorescent oligonucleotide probes for detecting protein-DNA interactions. Proc. Nat. Acad. Sci. USA. (2008), 105: 4156-4161.
- Cy7 (Cyanine 7)

Oligonucleotide Synthesis |  Flourescent Molecular Probes |  Gene Detection Systems |  Tools & Reagents |  Gene Assays |  RNAi
© 2017 Gene Link |  Terms & Conditions |  Licenses |  Privacy Policy |  June 25, 2017 11:58:35 PM