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IRDye 750-NHS

IRDye 750-N

Code : [IRD750-N]

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picture of IRDye 750-NHS

Modification : IRDye 750-N

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6648
Fluorescent Dyes
[IRD750-N]
Y
Y
Y
950
170
PS26-6672.pdf
756
776
-


Catalog NoScalePrice
26-6648-0550 nmol$728.00
26-6648-02200 nmol$728.00
26-6648-011 umol$985.00
26-6648-032 umol$1,382.00
26-6648-1010 umol$7,880.00
26-6648-1515 umol$9,850.00
26-6648-1515 umol$9,850.00
Discounts are available for IRDye 750-N!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

IR750-NHS is a near-IR fluorescent dye used for labeling oligonucleotides. IR750 has an absorbance maximum of 756 nm and an emission maximum of 776 nm. The combination of narrow absorbance/emission bands and low-background autofluorescence in the IR region results in higher S/N ratios and thus enhanced detection sensitivity compared with fluorophores with absorbance/emission maxima in the visible region (1). IRDye750 is used as a reporter moiety in real-time PCR applications. For such probes, IRDye750 is most commonly paired with the dark quencher QC-1, as the two have excellent spectral overlap (2).

IRDye750 can be used to label DNA oligos for use as hybridization probes in a variety of in vivo and in vitro research or diagnostic applications, as well as for structure-function studies of DNA, RNA, and protein-oligonucleotide complexes. Oligos labeled with IRDye750 at the 5-end can be used as PCR and Sanger DNA sequencing primers to generate fluorescently-labeled PCR, sequencing or genetic analysis (AFLP, microsatellite) products (3-5).


Near Infrared Fluorophore Spectral Data & Quencher Selection Guide

Fluorophore Name

Excitation Max, nm +/-10

Emission Max, nm +/-10

Extinction Coefficient*

Color**

Quencher

Cy5

650 665 250,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

IRDye 650 NHS

650 665 230,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

AZ647 NHS

655 680 191,800

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

Cy5.5

684 710 198,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

IRDye 700 NHS

684 710 288,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

Cy7 NHS

740 773 199,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

IRDye 750 NHS

756 776 260,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

cy7.5 NHS

788 808 223,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

IRDye 800 NHS

795 819 240,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

* Extinction coefficient at λ (max) in cm-1M-1. ** Typical emission color seen through the eyepiece of a conventional fluorescence microscope with appropriate filters. Near-IR region. Human vision is insensitive to light beyond ~650 nm; it is not possible to view near-IR fluorescent dyes.

Click here for a list of fluorophores.

Click here for list of quenchers.



References
1. Middendorf, L.R., Bruce, J.C., Eckles, R.D., Grone, D.L., Roemer, S.C., Sloniker, G.D., Steffens, D.L., Sutter, S.L., Brumbaugh, J.A., et al. Continuous, on-line DNA sequencing using a versatile infrared laser scanner/electrophoresis apparatus. Electrophoresis (1992), 13: 487-494.
2. Peng X., Chen, H., Draney, D.R., Volcheck, W., Schutz-Geschwender, A., Olive, D.M. A nonfluorescent, broad-range quencher dye for Forster resonance energy transfer assays. Anal. Biochem. (2009), 388: 220-228.
3. Yomano, L.P., Scopes, R.K., Ingram, L.O. Cloning, sequencing, and expression of the Zymomonas mobilis phosphoglycerate mutase gene (pgm) in Escherichia coli. J. Bacteriol. (1993), 175: 3926-3933.
4. Oetting, W.S., Lee, H.K., Flanders, D.J., Wiesner, T.A., King, R.A. Linkage Analysis with Multiplexed Short Tandem Repeat Polymorphisms Using Infrared Fluorescence and M13 Tailed Primers. Genomics (1995), 30: 450-458.
5. Myburg, A.A., Remington, D.L, OMalley, D.M., Sederoff, R.R., Whetton, R.W. High-Throughput AFLP Analysis Using Infrared Dye-Labeled Primers and an Automated DNA Sequencer. Biotechniques (2001), 30: 348-357.
- IRDye 750-NHS

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