IRDye QC-1 NHS Quencher

IRDye QC-1N Quencher

Code : [IRD-QC-1]

Modification : IRDye QC-1N Quencher

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6674
Quenchers
[IRD-QC-1]
Y
Y
Y
1145.67
-
PS26-6674.pdf
750
-
-

Catalog No	Scale	Price
26-6674-05	50 nmol	$486.00
26-6674-02	200 nmol	$702.00
26-6674-01	1 umol	$1,690.00
26-6674-03	2 umol	$3,105.00

Related Modifications
IRDye 700-N	IRDye 800-N

IRDye QC-1 quencher is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is required. A C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

YIELD
NHS based modifications are post synthesis conjugation performed using a primary amino group. The yield is lower as compared to direct automated coupling of modifications that are available as amidites. Approximate yield for various scales are given below.

~2 nmol final yield for 50 nmol scale synthesis.
~5 nmol final yield for 200 nmol scale synthesis.
~16 nmol final yield for 1 umol scale synthesis

IRDye QC-1 is a non-fluorescent (dark) quencher compatible with a wide range of visible and near-infrared fluorophores (~500-800 nm). It has a wide available quenching range with the maxima in the range of 700-800 nm. IRDye QC-1 quenches common fluorophores with >97% efficiency. It is ideal for all IR dyes. - IRDye QC-1 NHS Quencher