Quick Order |All Online Ordering|Product Catalog Ordering|Oligo Modifications List|Product Info & Literature|Oligo Design Tools/Resources

TaqMan - Molecular Beacons - Fluorescent Molecular Probes

Search Modifications

TaqMan probes and Molecular beacons with universal fluorescence quencher dabcyl or Tamra or BHQ at 3' end. Gene Link considers gel purification (PAGE) to be the gold standard method of purification and essential for optimum performance of fluorescent dye labeled oligonucleotides.Customers may request Molecular Beacons without gel purification for reduced pricing.

Special Pricing Fluorescent Probes

Price includes reverse phase purification. Guaranteed yield for dye and quencher listed.

Probe Type & Dye/Quencher

Price

Yield

5' 6-FAM/3' BHQ-1 $120.00 5 nmol
Hex, Tet, Cy3 or Cy5/3' BHQ-1 or BHQ-2 $230.00 5 nmol
Price includes synthesis and reverse phase purification of DNA oligo up to 32mer.
Additional per base cost above 32mer. Price subject to change without notice.

Fluorescent Probes Price List Summary* and PAGE Purified Yield**
 

50 nmol scale

200 nmol scale

1 umol scale

Dye Quencher

Price

Yield

Price

Yield

Price

Yield

5' 6-FAM and 3'-BHQ-1 or Tamra or Dabcyl $190.00 10 nmol $250.00 25 nmol $350.00 60 nmol

See above for special pricing for 5 nmol final RPC purified yield probes.

5'-Hex, Tet, Cy 3 or Cy 5/Quencher $230.00 5 nmol $290.00 15 nmol $450.00 40 nmol
Cy3.5, Cy5.5/Quencher $390.00 5 nmol $440.00 12 nmol $650.00 25 nmol
Cy7-NHS, 5'-NHS Dyes (Rox, AF Dyes, other)/Quencher $410.00 2 nmol $490.00 5 nmol $750.00 16 nmol
Other NHS Fluorophores/Quencher $490.00 2 nmol $510.00 5 nmol $790.00 16 nmol

*Price includes gel purification and does not include per base oligo synthesis charges. Royalty charges are additional. Pricing subject to change without notice.

**Approximate yield for dye and quencher listed. Lower yield with additional modifications.

Click here for a list of fluorophores.



Popular Fluorphores & Quenchers

Quencher & Fluorophore

Color

Suitable Quencher

Absorbance max (nm)

Emission max (nm)

Dabcyl (Quencher)     453 (380-530)  
BHQ-1 (Quencher)     534 (480-580)  
BHQ- 2 (Quencher)     579 (550-650)  
BHQ-3 (Quencher)     672 (620-730)  
BBQ-650 (Quencher)     650 (550-750)  
6-FAM (Fluorescein) Green BHQ-1 494 525
TET Orange BHQ-1 521 536
JOE Mustard BHQ-1 528 554
HEX Pink BHQ-1 535 556
Cy 3 Red BHQ-2 552 570
TAMRA Rose BHQ-2 565 580
Cy 3.5 Purple BHQ-2 588 604
ROX Purple BHQ-2 587 607
Cy 5 Violet BBQ-650 646 667
Cy 5.5 Blue BBQ-650 683 707
Cy 7 Near IR BBQ-650 743 767
More information on fluorescent dyes is available at this link. Fluorescent Dyes Reference & Price List

**Black Hole Quencher License Agreement
"Black Hole Quencher", "BHQ-1", "BHQ-2" and "BHQ-3" are registered trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ technology is licensed and sold under agreement with Biosearch and these products are sold exclusively for R&D use by the purchaser. They may not be used for clinical or diagnostic purposes and they may not be resold, distributed or re-packaged


Fluorophore Spectral Data & Quencher Selection Guide

Fluorophore Name

Excitation Max, nm

Emission Max, nm

Extinction Coefficient*

Color**

Quencher

AFDye-350 NHS

346

445

19,000

Blue

Dabcyl λ (max) = 453 nm Range = 380-530 nm

AFDye-405 NHS

402

424

33,000

Blue

Dabcyl λ (max) = 453 nm Range = 380-530 nm

PBlue-455 NHS

416

451

46,000

Blue

Dabcyl λ (max) = 453 nm Range = 380-530 nm

MBlue-460 NHS

362

459

20,000

Blue-Green

Dabcyl λ (max) = 453 nm Range = 380-530 nm

AFDye-488 NHS

494

517

73,000

Yellow-Green

BHQ-1 λ (max) = 534 nm Range = 480-580 nm

FAM

495

520

75,850

Yellow-Green

BHQ-1 λ (max) = 534 nm Range = 480-580 nm

TET

521

536

99,000

Yellow-Green

BHQ-1 λ (max) = 534 nm Range = 480-580 nm

AFDye-430 NHS

430

539

15,000

Yellow-Green

BHQ-1 λ (max) = 534 nm Range = 480-580 nm

Cal Fluor Gold 540

552

543

81,100

Yellow

BHQ-1 λ (max) = 534 nm Range = 480-580 nm

JOE

520

548

75,000

Yellow

BHQ-1 λ (max) = 534 nm Range = 480-580 nm

Yakima Yellow

531

549

83,800

Yellow

BHQ-1 λ (max) = 534 nm Range = 480-580 nm

AFDye-532 NHS

530

555

81,000

Yellow

BHQ-1 λ (max) = 534 nm Range = 480-580 nm

HEX

535

556

98,000

Yellow

BHQ-1 λ (max) = 534 nm Range = 480-580 nm

Cal Orange 560

537

558

150,000

Yellow

BHQ-2 λ (max) = 579 nm Range = 550-650 nm

CY3

550

570

150,000

Yellow-Orange

BHQ-2 λ (max) = 579 nm Range = 550-650 nm

AFDye-555 NHS

555

572

155,000

Yellow-Orange

BHQ-2 λ (max) = 579 nm Range = 550-650 nm

TAMRA

555

576

65,000

Yellow-Orange

BHQ-2 λ (max) = 579 nm Range = 550-650 nm

CAL Fluor Red 590

569

591

79,000

Yellow-Orange

BHQ-2 λ (max) = 579 nm Range = 550-650 nm

Redmond Red

579

595

52,300

Yellow-Orange

BHQ-2 λ (max) = 579 nm Range = 550-650 nm

CY3.5

581

596

150,000

Yellow-Orange

BHQ-2 λ (max) = 579 nm Range = 550-650 nm

ROX NHS

575

602

82,000

Orange

BHQ-2 λ (max) = 579 nm Range = 550-650 nm

AFDye-568 NHS

578

602

88,000

Orange

BHQ-2 λ (max) = 579 nm Range = 550-650 nm

Cal Red 610

590

610

108,000

Orange

BHQ-2 λ (max) = 579 nm Range = 550-650 nm

TXRed-616 NHS

595

615

80,000

Orange

BHQ-2 λ (max) = 579 nm Range = 550-650 nm

AFDye-594 NHS

590

617

92,000

Orange-Red

BHQ-2 λ (max) = 579 nm Range = 550-650 nm

CAL Fluor Red 635

616

637

112,000

Red

BBQ-650 λ (max) = 650nm Range = 550-750 nm

LC Red 640 NHS

625

640

110,000

Red

BBQ-650 λ (max) = 650nm Range = 550-750 nm

AFDye-647 NHS

649

671

270,000

Red

BBQ-650 λ (max) = 650nm Range = 550-750 nm

Cy5

649

670

250,000

Red

BBQ-650 λ (max) = 650nm Range = 550-750 nm

Cy5.5

675

694

190,000

Red

BBQ-650 λ (max) = 650nm Range = 550-750 nm

AFDye-680 NHS

678

701

185,000

Near-IR region. Human vision is insensitive to light beyond ~650 nm; it is not possible to view near-IR fluorescent dyes.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

Cy7 NHS

750

773

199,000

Near-IR region. Human vision is insensitive to light beyond ~650 nm; it is not possible to view near-IR fluorescent dyes.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

IR 750 NHS

756

776

260,000

Near-IR region. Human vision is insensitive to light beyond ~650 nm; it is not possible to view near-IR fluorescent dyes.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

Cy7.5 NHS

788

808

223,000

Near-IR region. Human vision is insensitive to light beyond ~650 nm; it is not possible to view near-IR fluorescent dyes.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

* Extinction coefficient at λ (max) in cm–1M–1. ** Typical emission color seen through the eyepiece of a conventional fluorescence microscope with appropriate filters.

Oligonucleotide Synthesis |  Flourescent Molecular Probes |  Gene Detection Systems |  Tools & Reagents |  Gene Assays |  RNAi
© 2021 Gene Link |  Terms & Conditions |  Licenses |  Privacy Policy |  May 7, 2021 5:18:45 PM