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In this section: Introduction | Molecular Beacon FAQ's | Fluorescent Probes Price List | Other Fluorescent Molecular Probes
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In this section: Gene Construction
In this section: Introduction | The Omni-Clean™ System | The Omni-Pure™ Plasmid Purification System | The Omni-Pure™ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
In this section: Introduction | Quality Control | Purification | Modifications | Long Oligos | Price List
In this section: Introduction | Molecular Beacon FAQ's | Fluorescent Probes Price List | Other Fluorescent Molecular Probes
In this section: SPCT | DME SPCT Intro | Order DME TaqMan® Assays SPCT | SNP PCT Search | Gene Expression Assays | SPCT Design Center | GeneAssays
In this section: RNA Oligonucleotides | Quality Control | Purification | Modifications | RNAi Explorer™ Products and Prices | Custom RNAi | RNAi Design Guidelines | SmartBase™ siRNA Modifications | shRNA Explorer™
In this section: PCR Amplification & Analysis
In this section: Introduction | Genemer™ | GeneProber™ | Prober™ Gene Detection Kits | GScan™ Gene Detection Kits | Genemer™ Control DNA | Infectious Diseases
In this section: Gene Construction
In this section: Introduction | The Omni-Clean™ System | The Omni-Pure™ Plasmid Purification System | The Omni-Pure™ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
Tris NTA (Tris Nitrilotriacetate) Oligo Conjugate
NTA Tris
Code : [NTA-Tris]
Modification : NTA Tris
Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC
26-6614
Affinity Ligands
[NTA-Tris]
Y
N
N
1049
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PS26-6614.pdf
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-
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Catalog No | Scale | Price | 26-6614-05 | 50 nmol | $510.00 | 26-6614-02 | 200 nmol | $510.00 | 26-6614-01 | 1 umol | $775.00 | 26-6614-03 | 2 umol | $1,285.00 | 26-6614-10 | 10 umol | $5,480.00 | 26-6614-15 | 15 umol | $7,185.00 |
Gene Link provides custom synthesis of NTA Tris (Nitrilotriacetate) Oligo Conjugate.
NTA-Tris modification is a post synthesis conjugation to an active NHS group of either a NHS-Carboxy C10 or a NHS-dT group thus an additional modification is required at the 5' end with additional charges for that modification.
YIELD
NHS based modifications are post synthesis conjugation performed, the yield is lower as compared to direct automated coupling of modifications that are available as amidites. Approximate yield for various scales are given below.
Yield given below are for oligos shorter than 50mer. Please see longer oligos yield at this link Long Oligo Typical Yield.
~2 nmol final yield for 50 nmol scale synthesis.
~5 nmol final yield for 200 nmol scale synthesis.
~16 nmol final yield for 1 umol scale synthesis
~32 nmol final yield for 2 umol scale synthesis
~160 nmol final yield for 10 umol scale synthesis
~240 nmol final yield for 15 umol scale synthesis
Mono-NTA has an affinity of ~10 uM towards His-tag
Tris-NTA (3 Ni-NTA) groups bind His-tag with an affinity of ~10 uM towards His-tag. This is 10.000-fold higher affinity as compared to mono-NTA.
Binding is reversible with imidazole or EDTA
Allows reversible labeling of proteins or cell surfaces
Immobilize proteins, lipids or cells to surfaces
Enables wide variety of functional assays
- Tris NTA (Tris Nitrilotriacetate) Oligo Conjugate
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