Antisense/ASO & siRNA - Introduction

Introduction to Antisense/ASO & siRNA

Antisense/ASO & siRNA Applications

Antisense/ASO & siRNA Design/Protocol

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Introduction to Antisense/ASO & siRNA

An antisense oligonucleotide refers to a short, synthetic DNA or RNA strand that is complementary in sequence to a short target sequence on a particular mRNA strand, which upon specific hybridization to its target induces inhibition of gene expression. The mechanism of inhibition is based on two properties: first, the physical blocking of the translation process by the presence of the short double-stranded region, and second, in the case of antisense DNA, the resulting DNA-RNA duplex is susceptible to cleavage by cellular RNase H activity, which degrades the mRNA and prevents proper translation. The latter property is the 'classic' mode of action for antisense oligos. The former property can be used when it is necessary to design an antisense oligo with certain modifications that result in it not supporting RNase-H activity (1,2).

Antisense/ASO & siRNA Modifications						View PDF	Excel Mods & Code List
Product	Code	Catalog No.	50 nmol scale (XX=05)	200 nmol scale (XX=02)	1 mmol scale (XX=01)	2 mmol scale (XX=03)	10 mmol scale (XX=10)	15 mmol scale (XX=15)
2'-Amino-C	[2-AmC]	27-6506	$270.00	$270.00	$335.00	$461.00	$729.00	$945.00
2'-Amino-U	[2-AmU]	27-6507	$270.00	$270.00	$335.00	$461.00	$729.00	$945.00
2'-Fluoro Bases	[fN]	26-6692N	$35.00	$35.00	$41.00	$59.00	$239.00	$309.00
2'-Fluoro deoxyadenosine (2'-F-A)	[fA]	26-6692	$42.00	$42.00	$50.00	$70.00	$265.00	$340.00
2'-fluoroarabinoside-A (FANA-A)	[FANA-A]	27-6605A	$281.00	$281.00	$319.00	$410.00	$659.00	$810.00
2'-fluoroarabinoside-Base (FANA-N)	[FANA-N]	27-6605N	$286.00	$286.00	$319.00	$410.00	$659.00	$810.00
2'-fluoroarabinoside-C (FANA-C)	[FANA-C]	27-6605C	$248.00	$248.00	$297.00	$335.00	$659.00	$810.00
2'-fluoroarabinoside-G (FANA-G)	[FANA-G]	27-6605G	$281.00	$281.00	$319.00	$410.00	$659.00	$810.00
2'-fluoroarabinoside-mU (FANA-mU)	[FANA-mU]	27-6605mU	$248.00	$248.00	$297.00	$335.00	$659.00	$810.00
2'-fluoroarabinoside-U (FANA-U)	[FANA-U]	27-6605U	$248.00	$248.00	$335.00	$335.00	$659.00	$810.00
2'-Fluoro deoxycytosine (2'-F-C)	[fC]	26-6463	$27.00	$27.00	$39.00	$49.00	$214.00	$321.00
2'-Fluoro deoxyguanosine (2'-F-G)	[fG]	26-6693	$42.00	$42.00	$50.00	$70.00	$265.00	$340.00
2'-F Inosine (2'-F-I)	[fI]	26-6618	$140.00	$140.00	$178.00	$213.00	$663.00	$848.00
2'-Fluoro deoxyuridine (2'-F-U)	[fU]	26-6462	$27.00	$27.00	$39.00	$49.00	$214.00	$321.00
2'-O-methoxy-ethyl Bases (2'-MOE)	[MOE-N]	27-6450N	$15.00	$15.00	$18.00	$28.00	$119.00	$149.00
2'-O-methoxy-ethyl 5me Cytidine-(2'-MOE meC)	[MOE-mC]	27-6450mC	$15.00	$15.00	$18.00	$28.00	$119.00	$149.00
2'-O-methoxy-ethyl 5me Uridine-(2'-MOE 5me U)	[MOE-mU]	27-6450mU	$15.00	$15.00	$18.00	$28.00	$119.00	$149.00
2'-O-methoxy-ethyl Adenosine-(2'-MOE rA)	[MOE-A]	27-6450A	$15.00	$15.00	$18.00	$28.00	$119.00	$149.00
2'-O-methoxy-ethyl Guanosine-(2'-MOE rG)	[MOE-G]	27-6450G	$15.00	$15.00	$18.00	$28.00	$119.00	$149.00
2'-O Me-5-Me-C	[m5mC]	27-6508	$286.00	$286.00	$341.00	$460.00	$1,393.00	$1,741.00
2'-O methyl adenosine A	[mA]	27-6410A	$9.72	$9.72	$19.00	$30.00	$110.00	$160.00
2'-O methyl bases	[mN]	27-6410N	$9.72	$9.72	$19.00	$30.00	$110.00	$160.00
2'-O methyl cytosine C	[mC]	27-6410C	$9.72	$9.72	$19.00	$30.00	$110.00	$160.00
2'-O methyl guanosine G	[mG]	27-6410G	$8.64	$9.72	$19.00	$30.00	$110.00	$160.00
2'-O methyl Inosine	[mI]	27-6410I	$211.00	$211.00	$274.00	$411.00	$2,190.00	$2,738.00
2'-O methyl uridine U	[mU]	27-6410U	$9.72	$9.72	$19.00	$30.00	$110.00	$160.00
3'-O methyl rA (2'-5' linked)	[3OmA]	27-6458A	$227.00	$227.00	$297.00	$335.00	$2,376.00	$2,970.00
3'-O methyl rC (2'-5' linked)	[3OmC]	27-6458C	$227.00	$227.00	$297.00	$335.00	$2,376.00	$2,970.00
3'-O methyl rG (2'-5' linked)	[3OmG]	27-6458G	$227.00	$227.00	$297.00	$335.00	$2,376.00	$2,970.00
3'-O methyl rInosine (2'-5' linked)	[3OmI]	27-6458I	$321.00	$321.00	$373.00	$443.00	$2,560.00	$3,094.00
3'-O methyl rU (2'-5' linked)	[3OmU]	27-6458U	$227.00	$227.00	$297.00	$335.00	$2,376.00	$2,970.00
3'-rA (2'-5' linked)	[3rA2-5]	26-6682	$410.00	$410.00	$575.00	$748.00	$3,987.00	$4,984.00
3'-rC (2'-5' linked)	[3rC2-5]	26-6911	$410.00	$410.00	$575.00	$748.00	$3,987.00	$4,984.00
3'-rG (2'-5' linked)	[3rG2-5]	26-6912	$410.00	$410.00	$575.00	$748.00	$3,987.00	$1,241.00
3'-rU (2'-5' linked)	[3rU2-5]	26-6913	$410.00	$410.00	$575.00	$748.00	$3,987.00	$1,241.00
5-methyl deoxycytosine [5mdC]	[5mdC]	26-6413	$67.00	$67.00	$82.00	$145.00	$473.00	$629.00
Alpha Tocopherol TEG (Vitamin E)	[a-toco-TEG]	26-6616	$319.00	$319.00	$405.00	$486.00	$1,075.00	$1,312.00
Alpha-linolyl (C18:3α) ALA LMO	[3-ALA-C18-3]	26-6784	$254.00	$286.00	$335.00	$394.00	$2,133.00	$2,500.00
Arachidonyl (C20:4) AA LMO	[3-AA-C20-4]	26-6787	$254.00	$286.00	$335.00	$394.00	$2,133.00	$2,500.00
butyric acid (C4) Modified Oligo	[3-BA-C4]	26-6782	$120.00	$120.00	$150.00	$225.00	$850.00	$1,200.00
Cholesterol TEG (15 atom triethylene glycol spacer)	[CholTEG]	26-6602	$235.00	$235.00	$265.00	$325.00	$1,066.00	$1,361.00
Cholesterol TEG-3' (15 atom triethylene glycol spacer)	[3-CholTEG]	26-6570	$189.00	$189.00	$227.00	$286.00	$1,066.00	$1,361.00
Docosanoic (C22) DCA LMO	[3-DCA-C22]	26-6627	$254.00	$286.00	$335.00	$394.00	$2,133.00	$2,500.00
Docosahexaenoic (C22:6) DHA LMO	[3-DHA-C22-6]	26-6626	$254.00	$286.00	$335.00	$394.00	$2,133.00	$2,500.00
Dihomo-gamma-linolyl (C20:3) DGLA LMO	[3-DGLA-C20-3]	26-6786	$254.00	$286.00	$335.00	$394.00	$2,133.00	$2,500.00
Eicosapent (20:5) EPA LMO	[3-EPA-C20-5]	26-6788	$254.00	$286.00	$335.00	$394.00	$2,133.00	$2,500.00
GalNAc Oligo N-Acetylgalactosamine C3	[GalNAc]	26-6751	$416.00	$416.00	$443.00	$490.00	$1,388.00	$1,630.00
GalNAc Trivalent TEG	[GalNAc-TEG3X]	26-6735	$443.00	$443.00	$502.00	$583.00	$3,262.00	$4,018.00
Gamma-linolyl (C18:3γ) GLA LMO	[3-GLA-C18-3]	26-6785	$254.00	$286.00	$335.00	$394.00	$2,133.00	$2,500.00
Lignoceryl C24 (3') LMO	[3-Lig-C24]	26-6624	$513.00	$610.00	$848.00	$1,782.00	$4,914.00	$6,113.00
Lignoceryl C24 (5') LMO	[5-Lig-C24]	26-6625	$513.00	$610.00	$848.00	$1,109.00	$4,914.00	$6,113.00
Linolyl (C18:2) LA LMO	[3-LA-C18-2]	26-6783	$254.00	$286.00	$335.00	$394.00	$2,133.00	$2,500.00
Locked Analog A (LNA-A)	[+A]	26-6517	$41.00	$41.00	$49.00	$81.00	$340.00	$443.00
Locked Analog Bases (LNA-N)	[+N]	26-6517N	$41.00	$41.00	$49.00	$81.00	$340.00	$443.00
Locked Analog G (LNA-G)	[+G]	26-6518	$41.00	$41.00	$49.00	$81.00	$340.00	$443.00
Locked Analog mC (LNA-mC)	[+mC]	26-6519	$41.00	$41.00	$49.00	$81.00	$340.00	$443.00
Locked Analog T (LNA-T)	[+T]	26-6520	$41.00	$41.00	$49.00	$81.00	$340.00	$443.00
Mesyl Phosphoramidate Linkages (Ms, u)	[Ms]	26-6914	$92.00	$92.00	$147.00	$231.00	$706.00	Inquire
Methyl Phosphonate	[mp-dN]	26-6510	$70.00	$70.00	$91.00	$137.00	$730.00	$913.00
Methyl Phosphonate dA [mp-dA]	[mp-dA]	26-6611A	$70.00	$70.00	$92.00	$119.00	$680.00	$880.00
Methyl Phosphonate dC [mp-dC]	[mp-dC]	26-6611C	$70.00	$70.00	$92.00	$119.00	$680.00	$880.00
Methyl Phosphonate dG [mp-dG]	[mp-dG]	26-6611G	$70.00	$70.00	$92.00	$119.00	$680.00	$880.00
Methyl Phosphonate dT [mp-dT]	[mp-dT]	26-6611T	$70.00	$70.00	$92.00	$119.00	$680.00	$880.00
Morpholino Phosphoroamidate Oligo A [MO-A]	[MO-A]	26-6644A	$210.00	$210.00	$265.00	$365.00	$810.00	$1,110.00
Morpholino Phosphoroamidate Oligo C [MO-C]	[MO-C]	26-6644C	$210.00	$210.00	$265.00	$365.00	$810.00	$1,110.00
Morpholino Phosphoroamidate Oligo G [MO-G]	[MO-G]	26-6644G	$210.00	$210.00	$265.00	$365.00	$810.00	$1,110.00
Morpholino Phosphoroamidate Oligo T [MO-T]	[MO-T]	26-6644T	$210.00	$210.00	$265.00	$365.00	$810.00	$1,110.00
Ethoxy Phosphate dA	[EoP-dA]	26-6641A	$90.00	$90.00	$116.00	$145.00	$680.00	$880.00
Ethoxy Phosphate dC	[EoP-dC]	26-6641C	$90.00	$90.00	$116.00	$145.00	$680.00	$880.00
Ethoxy Phosphate dG	[EoP-dG]	26-6641G	$90.00	$90.00	$116.00	$145.00	$680.00	$880.00
Ethoxy Phosphate dT	[EoP-dT]	26-6641T	$90.00	$90.00	$116.00	$145.00	$680.00	$880.00
Methoxy Phosphate dA	[MoP-dA]	26-6642A	$90.00	$90.00	$116.00	$145.00	$680.00	$880.00
Methoxy Phosphate dC	[MoP-dC]	26-6642C	$90.00	$90.00	$116.00	$145.00	$680.00	$880.00
Methoxy Phosphate dG	[MoP-dG]	26-6642G	$90.00	$90.00	$115.00	$145.00	$680.00	$880.00
Methoxy Phosphate dT	[MoP-dT]	26-6642T	$90.00	$90.00	$116.00	$145.00	$680.00	$880.00
Palmityl C16 (3') LMO	[3-Pal-C16]	26-6621	$513.00	$610.00	$848.00	$1,109.00	$4,914.00	$6,113.00
Palmityl C16 (5') LMO	[5-Pal-C16]	26-6622	$513.00	$610.00	$848.00	$1,109.00	$4,914.00	$6,113.00
Palmityl C16 Oligo (NHS) LMO	[Pal-C16-N]	26-6619	$513.00	$610.00	$1,042.00	$1,782.00	$4,914.00	$6,113.00
Phosphorothioate (SOX)	[Ps]	26-6401	$3.78	$4.59	$7.02	$9.72	$56.00	$70.00
Phosphorylation-3'	[3Phos]	26-6427	$59.00	$59.00	$119.00	$216.00	$950.00	$1,188.00
Phosphorylation-5'	[5Phos]	26-6428	$22.00	$27.00	$49.00	$73.00	$194.00	$292.00
Propionyl (C3) PA LMO	[3-PA-C3]	26-6781	$120.00	$120.00	$150.00	$225.00	$850.00	$1,200.00
Spermine Oligo Cationic Tail (ZNA-Oligos)	[Spm]	26-6454	$350.00	$350.00	$455.00	$682.00	$3,639.00	$4,549.00
Stearyl C18 LMO LMO 3'	[3-Str-C18]	26-6623	$200.00	$200.00	$255.00	$362.00	$1,782.00	$2,144.00
Stearyl C18 LMO Oligo 5'	[5-Str-C18]	26-6617	$81.00	$81.00	$119.00	$200.00	$556.00	$729.00
Thio-Morpholino Oligo A [TMO-A]	[TMO-A]	26-6645A	$210.00	$210.00	$265.00	$365.00	$810.00	$1,110.00
Thio-Morpholino Oligo C [TMO-C]	[TMO-C]	26-6645C	$210.00	$210.00	$265.00	$365.00	$810.00	$1,110.00
Thio-Morpholino Oligo G [TMO-G]	[TMO-G]	26-6645G	$210.00	$210.00	$265.00	$365.00	$810.00	$1,110.00
Thio-Morpholino Oligo T [TMO-T]	[TMO-T]	26-6645T	$210.00	$210.00	$265.00	$365.00	$810.00	$1,110.00
Vitamin B12 (Cyanocobalamin) Oligo	[Vit-B12-N]	26-6572	$373.00	$632.00	$752.00	$956.00	$5,288.00	$7,932.00
vP-mU 5'-Vinylphosphonate 2'O mU	[vP-mU]	26-6597	$254.00	$254.00	$308.00	$340.00	$815.00	$1,064.00

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Antisense/ASO & siRNA - Introduction

Introduction to Antisense/ASO & siRNA

Antisense/ASO & siRNA Modifications