FISH Probes - Introduction

Introduction to FISH Probes

FISH Probes Applications

FISH Probes Design/Protocol

FISH Probes Literature

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Introduction to FISH Probes

Probes for fluorescent in situ hybridization (FISH) are derived from repetitive chromosomal regions to paint a chromosome or are directed towards unique gene(s) for identification. These probes are usually developed from clones (BAC and cosmids) and more recently synthesized chemically as oligonucleotides appropriately labeled with fluorescent dyes. The oligo probes can be synthesized as tiles or longer sequences up to 250mer in length by Gene Link. Gene Link presents various design options for synthesizing �Smart� FISH probes. Smart FISH probes can be synthesized in a predetermined way to exhibit the features that is desired; for instance to increase nuclease resistance we can substitute standard bases with 2� Fluoro bases. Standard DNA probes are like natural molecules that are prone to degradation under normal conditions, ubiquitous nucleases as well as chemical instability lead to fast degradation with a finite half life. The premise of this product guide is to discuss various modifications that are offered by Gene Link that may be used for design and synthesis of Smart FISH Probes. There are four common features that are desirable and in particular can be improved by using a combination of available nucleic acid modifications that modify the phosphodiester linkages, nucleic acid bases, the sugar moieties and addition of various other functional groups.

FISH Probes Modifications						View PDF	Excel Mods & Code List
Product	Code	Catalog No.	50 nmol scale (XX=05)	200 nmol scale (XX=02)	1 mmol scale (XX=01)	2 mmol scale (XX=03)	10 mmol scale (XX=10)	15 mmol scale (XX=15)
2'-Fluoro deoxyadenosine (2'-F-A)	[fA]	26-6692	$42.00	$42.00	$50.00	$70.00	$265.00	$340.00
2'-Fluoro deoxycytosine (2'-F-C)	[fC]	26-6463	$27.00	$27.00	$39.00	$49.00	$214.00	$321.00
2'-Fluoro deoxyguanosine (2'-F-G)	[fG]	26-6693	$42.00	$42.00	$50.00	$70.00	$265.00	$340.00
2'-Fluoro deoxyuridine (2'-F-U)	[fU]	26-6462	$27.00	$27.00	$39.00	$49.00	$214.00	$321.00
2'-O Me-5-Me-C	[m5mC]	27-6508	$286.00	$286.00	$341.00	$460.00	$1,393.00	$1,741.00
2'-O methyl adenosine A	[mA]	27-6410A	$9.72	$9.72	$19.00	$30.00	$110.00	$160.00
2'-O methyl cytosine C	[mC]	27-6410C	$9.72	$9.72	$19.00	$30.00	$110.00	$160.00
2'-O methyl guanosine G	[mG]	27-6410G	$8.64	$9.72	$19.00	$30.00	$110.00	$160.00
2'-O methyl uridine U	[mU]	27-6410U	$9.72	$9.72	$19.00	$30.00	$110.00	$160.00
2-Amino dA	[Am-dA]	26-6525	$176.00	$176.00	$228.00	$342.00	$1,825.00	$2,282.00
Cy3-5' (Cyanine 3)	[5-Cy3]	26-6437	$136.00	$136.00	$185.00	$245.00	$1,011.00	$1,264.00
Cy5 (5'-Cyanine 5)	[5-Cy5]	26-6436	$155.00	$155.00	$195.00	$245.00	$1,092.00	$1,366.00
5-methyl deoxycytosine [5mdC]	[5mdC]	26-6413	$67.00	$67.00	$82.00	$145.00	$473.00	$629.00
Amino C6 U (RNA)	[AmC6U]	27-6422	$875.00	$875.00	$910.00	$2,110.00	$4,421.00	$6,336.00
Amino deoxyadenosine dA C6	[Am-dA-C6]	26-6666	$267.00	$267.00	$298.00	$344.00	$1,717.00	$2,404.00
Amino deoxycytosine dC C6	[Am-dC-C6]	26-6670	$267.00	$267.00	$298.00	$344.00	$1,717.00	$2,404.00
Amino deoxyguanosine dG C6	[Am-dG-C6]	26-6669	$518.00	$518.00	$551.00	$594.00	$2,538.00	$3,408.00
Amino deoxythymidine dT C6	[Am-dT-C6]	26-6438	$199.00	$199.00	$228.00	$297.00	$1,485.00	$1,782.00
Cy3.5 (Cyanine 3.5)	[Cy3.5]	26-6461	$713.00	$713.00	$927.00	$1,390.00	$7,413.00	$9,266.00
Cy5.5 (Cyanine 5.5)	[Cy5.5]	26-6460	$486.00	$486.00	$632.00	$948.00	$5,054.00	$6,318.00
Cy7 (Cyanine 7)	[Cy7-N]	26-6474	$351.00	$351.00	$457.00	$685.00	$3,653.00	$4,566.00
Fluorescein dT (Fam dT)	[Fam-dT]	26-6422	$340.00	$340.00	$421.00	$486.00	$3,370.00	$4,860.00
Fluorescein-5'	[Fl-5]	26-6426	$68.00	$68.00	$88.00	$133.00	$708.00	$885.00
Hex-5' (Hexachloro-Fluorescein)	[5-Hex]	26-6432	$115.00	$115.00	$185.00	$235.00	$708.00	$885.00
Tet-5' (Tetrachloro-Fluorescein)	[Tet-5]	26-6433	$97.00	$97.00	$146.00	$219.00	$1,166.00	$1,458.00

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FISH Probes - Introduction

Introduction to FISH Probes

FISH Probes Modifications