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FISH Probes Applications

Introduction to FISH Probes FISH Probes Applications FISH Probes Design/Protocol FISH Probes Literature Order Online

FISH Probes Applications

Probes for fluorescent in situ hybridization (FISH) are derived from repetitive chromosomal regions to paint a chromosome or are directed towards unique gene(s) for identification. These probes are usually developed from clones (BAC and cosmids) and more recently synthesized chemically as oligonucleotides appropriately labeled with fluorescent dyes. The oligo probes can be synthesized as tiles or longer sequences up to 250mer in length by Gene Link.

References

1. Milligan, J.F., Matteucci, M.D. and Martin, J.C. (1993) Current concepts in antisense drug design. J. Medicinal Chem. 36:1923-1937.
2. Helene, C., Toulme, J. (1990) Specific regulation of gene expression by antisense, sense and antigene nucleic acids. Biochim. Biophys. Acta. 1049: 99-125.
3. Weintraub, H. M. (1990) Antisense RNA and DNA. Sci. Amer. 262:40-46.
4. Iyer, R.P., Egan. W., Regan, J.B and Beaucage, S.L. (1990) J. Am. Chem. Soc.112; 1253-1254.
5. Wagner, R.W., Matteucci, M.D., Lewis, J.G., Gutierrez, A.J., Moulds, C. and Froehler, B.C. (1993) Antisense gene inhibition by oligonucleotides containing C-5 propyne pyrimidines. Science 260:1510-1513.
6. Hertoghs, K.M.L., Ellis, J.H. and Catchpole, I.R (2003) Use of locked nucleic acid oligonucleotides to add functionality to plasmid DNA. Nucl. Acids Res.31 (20): 5817-5830.
7. Cotton, M., Oberhauser, B., Burnar, H. et al. (1991) 2'O methyl and 2'O ethyl oligoribonucleotides as inhibitors of the in vitro U7 snRNPdependent messenger-RNA processing event. NAR 19:2629.
8. Singh, S.K., Nielsen, P., Koshkin, A.A. and J. Wengel, Chem. Comm., 1998, (4), 455-456.
9. A.A. Koshkin, A.A., Singh, S.K., Nielsen, P., Rajwanshi, V.K., Kumar, R., Meldgaard, M., Olsen, C.E and Wengel, J. (1998) Tetrahedron 54:3607-3630.
10. Kværnø, L. and Wengel, J. (1999) Chem. Comm., 7:657-658.
11. Petersen, M and Wengel, J. (2003) Trends in Biotechnology 21(2): 74-81.
12. P.A. Giannaris, P.A. and Damha, M.J (1993) Nucleic Acids Research, 21:4742-4749.
13. Bhan, P., Bhan, A., Hong, M.K., Hartwell, J.G., Saunders, J.M and Hoke, G.D (1997) Nucleic Acids Res, 25:3310-3317.
14. Coleman, R.S. and Kesicki, E.S (1994) J. Amer. Chem. Soc., 116:11636-11642. 15. John SantaLucia, Jr. (1998) Proc. Natl. Acad. Sci. 95 1460-1465.

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