In this section: Introduction | Quality Control | Purification | Modifications | Long Oligos | Price List
In this section: Introduction | Molecular Beacon FAQ's | Fluorescent Probes Price List | Other Fluorescent Molecular Probes
In this section: SPCT | DME SPCT Intro | Order DME TaqMan® Assays SPCT | SNP PCT Search | Gene Expression Assays | SPCT Design Center | GeneAssays
In this section: RNA Oligonucleotides | Quality Control | Purification | Modifications | RNAi Explorerâ„¢ Products and Prices | Custom RNAi | RNAi Design Guidelines | SmartBaseâ„¢ siRNA Modifications | shRNA Explorerâ„¢
In this section: PCR Amplification & Analysis
In this section: Introduction | Genemerâ„¢ | GeneProberâ„¢ | Proberâ„¢ Gene Detection Kits | GScanâ„¢ Gene Detection Kits | Genemerâ„¢ Control DNA | Infectious Diseases
In this section: Gene Construction
In this section: Introduction | The Omni-Cleanâ„¢ System | The Omni-Pureâ„¢ Plasmid Purification System | The Omni-Pureâ„¢ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
In this section: Introduction | Quality Control | Purification | Modifications | Long Oligos | Price List
In this section: Introduction | Molecular Beacon FAQ's | Fluorescent Probes Price List | Other Fluorescent Molecular Probes
In this section: SPCT | DME SPCT Intro | Order DME TaqMan® Assays SPCT | SNP PCT Search | Gene Expression Assays | SPCT Design Center | GeneAssays
In this section: RNA Oligonucleotides | Quality Control | Purification | Modifications | RNAi Explorerâ„¢ Products and Prices | Custom RNAi | RNAi Design Guidelines | SmartBaseâ„¢ siRNA Modifications | shRNA Explorerâ„¢
In this section: PCR Amplification & Analysis
In this section: Introduction | Genemerâ„¢ | GeneProberâ„¢ | Proberâ„¢ Gene Detection Kits | GScanâ„¢ Gene Detection Kits | Genemerâ„¢ Control DNA | Infectious Diseases
In this section: Gene Construction
In this section: Introduction | The Omni-Cleanâ„¢ System | The Omni-Pureâ„¢ Plasmid Purification System | The Omni-Pureâ„¢ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
2'-O-methoxy-ethyl Adenosine-(2'-MOE rA)
Modification : 2'-MOE-A
Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC
Catalog No | Scale | Price | 27-6450A-05 | 50 nmol | $14.25 | 27-6450A-02 | 200 nmol | $14.25 | 27-6450A-01 | 1 umol | $16.50 | 27-6450A-03 | 2 umol | $26.00 | 27-6450A-10 | 10 umol | $110.00 | 27-6450A-15 | 15 umol | $138.00 |
Discounts are available for 2'-MOE-A! |
Modification* Discount Price Structure |
1 site/order
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List price
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2 sites/order
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10% discount
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3 sites/order
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20% discount
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4 sites/order
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30% discount
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5-9 sites/order
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50% discount
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10+ sites/order
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60% discount
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*Exceptions apply
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Antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) are both recognized therapeutic agents for the silencing of specific genes at the posttranscriptional level. Chemical modifications, particularly 2’-O-(2-Methoxyethyl)- oligoribonucleotides (2’-O-MOE bases) and 2’-O-Methyl bases are commonly used to confer nuclease resistance to an oligo designed for anti-sense, siRNA or aptamer-based research, diagnostic or therapeutic purposes, when specific 2’-OH is not required.
Nuclease resistance can be further enhanced by phosphorothiolation of appropriate phosphodiester linkages within the oligo. These modifications confers nuclease resistance, high binding affinity towards complementary RNA, reduced unspecific protein binding and extended half-life in tissues.
Gapmers. Currently, the mainstream of the ASO is gapmer design ASOs. Gapmer design oligonucleotides, contain two to
five chemically modified nucleotides (LNA, 2’-O methyl or 2’-O-MOE RNA) as “wings” at each terminus flanking a central 5- to10-base “gap” of DNA, enable cleavage of the target mRNA by RNase H, which recognizes DNA/RNA heteroduplexes. Usually all the phosphodiester linkages are converted to phosphorothioate.
Delivery. The development of effective delivery systems for antisense oligonucleotides is essential for their clinical therapeutic application. The most common delivery system involves a relatively hydrophobic molecule that can cross the lipid membrane. The following list of modifications are suitable for delivery system in addition to cell penetrating peptides.
Cholesterol
Tocopherol (alpha-tocopherol, a natural isomer of vitamin E)
PEG
- 2'-O-methoxy-ethyl Adenosine-(2'-MOE rA)
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