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dideoxy C-3'

picture of dideoxy C-3'

Modification : ddC-3'

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6337
End Blockers
3ddC
N
Y
N
273.19
7.4
PS26-6337.pdf
-
-
-


Catalog NoScalePrice
26-6337-0550 nmol$195.00
26-6337-02200 nmol$195.00
26-6337-011 umol$292.00
26-6337-032 umol$438.50
26-6337-1010 umol$2,336.00
26-6337-1515 umol$2,920.00

Dideoxycytosine (ddC) is a dideoxyribonucleoside, and is a synthetic analog of deoxycytosine. The difference between the two is that, in ddC, both the 2- and 3-positions of the ribose have a hydrogen (-H) group substituted for the OH group, whereas in dC, only the 2-position is so substituted.

ddC is generally used in two applications. First, to block the 3-end of oligonucleotides to prevent their extension by a polymerase in a PCR reaction or PCR-based assay. For example, 3-ddC-blocked oligos have been used in microarray-based DNA re-sequencing (1). ddC is also used to block the 3-end of 5-adenylated oligos (5-App) that are used as adapters in miRNA library generation. Blocking the 3-end in this manner prevents the oligo from either circularization (by self-ligation) or concatemerization to other 5-App oligos (2).

References
1. Sram, J., Sommer, S.S., Liu, Q. Microarray-based DNA re-sequencing using 3 blocked primers. Anal. Biochem. (2008), 3744: 41-47.
2. Lau, N.C., Lim, L.P., Weinstein, E.G., Bartel, D.P. An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans.Science (2001), 294: 858-862.
- dideoxy C-3'

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