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In this section: Gene Construction
In this section: Introduction | The Omni-Clean™ System | The Omni-Pure™ Plasmid Purification System | The Omni-Pure™ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
In this section: Introduction | Quality Control | Purification | Modifications | Long Oligos | Price List
In this section: Introduction | Molecular Beacon FAQ's | Fluorescent Probes Price List | Other Fluorescent Molecular Probes
In this section: SPCT | DME SPCT Intro | Order DME TaqMan® Assays SPCT | SNP PCT Search | Gene Expression Assays | SPCT Design Center | GeneAssays
In this section: RNA Oligonucleotides | Quality Control | Purification | Modifications | RNAi Explorer™ Products and Prices | Custom RNAi | RNAi Design Guidelines | SmartBase™ siRNA Modifications | shRNA Explorer™
In this section: PCR Amplification & Analysis
In this section: Introduction | Genemer™ | GeneProber™ | Prober™ Gene Detection Kits | GScan™ Gene Detection Kits | Genemer™ Control DNA | Infectious Diseases
In this section: Gene Construction
In this section: Introduction | The Omni-Clean™ System | The Omni-Pure™ Plasmid Purification System | The Omni-Pure™ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
3'-O-propargyl/Alkyne C 2'-5' linked
3'-O-propargyl C 2'-5' linked
Code : [PPG-3-O-C]
Modification : 3'-O-propargyl C 2'-5' linked
Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC
27-6919C
Click Chemistry
[PPG-3-O-C]
Y
Y
Y
330.2
7.2
PS27-6919C.pdf
260
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Catalog No | Scale | Price | 27-6919C-05 | 50 nmol | $310.00 | 27-6919C-02 | 200 nmol | $310.00 | 27-6919C-01 | 1 umol | $375.00 | 27-6919C-03 | 2 umol | $548.00 | 27-6919C-06 | 5 umol | $1,426.00 | 27-6919C-10 | 10 umol | $2,376.00 | 27-6919C-15 | 15 umol | $2,970.00 |
Discounts are available for 3'-O-propargyl C 2'-5' linked! |
Modification* Discount Price Structure |
1 site/order
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List price
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2 sites/order
|
10% discount
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3 sites/order
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20% discount
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4 sites/order
|
30% discount
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5-9 sites/order
|
50% discount
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10+ sites/order
|
60% discount
|
*Exceptions apply
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Click here for a complete list of Click Chemistry Oligo Modifications
Propargyl refers to triple/alkyne bond structure next to a saturated position with the following structure HC≡C−CH2−,.
Placing a propargyl group at the 3' end in conjunction with an azide at the 5' position can be ligated using click chemistry.
Ligation of an oligo containing a 5'-azide with an oligo containing a 3'-propargyl group using Click Chemistry leads to a triazole linkage that has been shown to have in vivo biocompatibility. This technique has been used to synthesize DNA constructs up to 300 bases in length. When the resultant triazole linkage was placed in a PCR template, various polymerases were able to copy the sequence correctly. The linkage has also been shown to be compatible with transcription and rolling circle amplification, as well as gene expression in E. coli. In the RNA world, a hammerhead ribozyme containing the triazole linkage at the substrate cleavage site has been shown to retain its activity. A large variety of applications is envisaged for this biocompatible chemical ligation.
An azide can be introduced at the 5' end of an oligo using Iodo-dT (5'); catalog number. 26-6926.
- 3'-O-propargyl/Alkyne C 2'-5' linked
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