Modification : DBCO PEG4 NHS
Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC
26-6745
Click Chemistry
[DBCO-PEG4-N]
Y
Y
Y
551.68
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PS26-6745.pdf
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Catalog No | Scale | Price |
26-6745-05 | 50 nmol | $308.00 |
26-6745-02 | 200 nmol | $308.00 |
26-6745-01 | 1 umol | $394.00 |
26-6745-03 | 2 umol | $513.00 |
26-6745-06 | 5 umol | $1,773.00 |
26-6745-10 | 10 umol | $1,348.00 |
26-6745-15 | 15 umol | $1,685.00 |
This modification is a post synthesis NHS conjugation to a primary amino group thus an additional modification with an amino group is required. A C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.
This modification is a post synthesis NHS conjugation to a primary amino group thus an additional modification with an amino group is required. A C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.
YIELD
NHS based modifications are post synthesis conjugation performed using a primary amino group. The yield is lower as compared to direct automated coupling of modifications that are available as amidites. Approximate yield for various scales are given below.
~2 nmol final yield for 50 nmol scale synthesis.
~5 nmol final yield for 200 nmol scale synthesis.
~16 nmol final yield for 1 umol scale synthesis
~160 nmol final yield for 10 umol scale synthesis
~240 nmol final yield for 15 umol scale synthesis
Click here for a complete list of Click Chemistry Oligo Modifications
Cyclooctyne-based (dibenzocyclooctynes, DBCO) modifications offers the ease of copper-free click reagents. These are simple to use and has excellenet click performance in 17 hours or less at room temperature. Gene Link offers 5'-DBCO-TEG for preparing oligos with 5'-DBCO and a 15 tom triethylene glycol spacer arm, DBCO-dT for inserting a DBCO group at any position within the oligonucleotide and DBCO-sulfo-NHS Ester is also offered for post-synthesis conjugation reactions. DBCO-modified oligos may be conjugated with azides in organic solvents, such as DMSO, or aqeous buffers. Depending on the azide used, the reaction will go to completion in 4-17 hours at room temperature.
- DBCO PEG4 NHS