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DBCO Photocleavable NHS

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Modification : DBCO PC NHS

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6744
Click Chemistry
[DBCO PC N]
Y
Y
Y
-
-
PS26-6744.pdf
-
-
-


Catalog NoScalePrice
26-6744-0550 nmol$285.00
26-6744-02200 nmol$285.00
26-6744-011 umol$365.00
26-6744-032 umol$475.00
26-6744-1010 umol$1,248.00
26-6744-1515 umol$1,560.00

This modification is a post synthesis NHS conjugation to a primary amino group thus an additional modification with an amino group is required. A C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

Click here for a complete list of Click Chemistry Oligo Modifications



Photocleavable DBCO-NHS ester contains a spacer arm containing a photocleavable moiety, this can be efficiently photoreleased, typically >90% in 5-25 minutes using an near-UV low intensity lamp (e.g. 365 nm lamp at 1-5 mW/cm2).
Cyclooctyne-based modifications offers the ease of copper-free click reagents. These are simple to use and has excellenet click performance in 17 hours or less at room temperature. Gene Link offers 5'-DBCO-TEG for preparing oligos with 5-DBCO and a 15 tom triethylene glycol spacer arm, DBCO-dT for inserting a DBCO group at any position within the oligonucleotide and DBCO-sulfo-NHS Ester is also offered for post-synthesis conjugation reactions. DBCO-modified oligos may be conjugated with azides in organic solvents, such as DMSO, or aqeous buffers. Depending on the azide used, the reaction will go to completion in 4-17 hours at room temperature.


Photo Cleavage Protocol

Cleavage occurs by irradiation with near-UV light (300-350 nm, >90% cleavage occurs within 5-25 minutes. Try using a Black Ray XX-15 UV lamp (Ultraviolet Products Inc., San Gabriel, CA) at a distance of 15 cm (emission peak 365 nm, 300 nm cut-off, 1.1 mW intensity at~31 cm).




- DBCO Photocleavable NHS

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