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dideoxy A 5'

picture of dideoxy A 5'

Modification : ddA-5'

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6331
End Blockers
ddA-5'
Y
N
N
297.21
15.4
PS26-6331.pdf
-
-
-


Catalog NoScalePrice
26-6331-0550 nmol$195.00
26-6331-02200 nmol$195.00
26-6331-011 umol$292.00
26-6331-032 umol$438.00
26-6331-1010 umol$2,336.00
26-6331-1515 umol$2,920.00
Discounts are available for ddA-5'!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Dideoxyadenosine (ddA) is a dideoxyribonucleoside that can only be used to block the 5' end. Use ddC-3' or Spacer C3 to block the 3' end from polymerase extension. ddA is a synthetic analog of deoxyadenosine, in ddA, both the 2- and 3-positions of the ribose have a hydrogen (-H) group substituted for the OH group, whereas in dA, only the 2-position is so substituted. ddA is added to the 5-end of an oligo via 5-to-5 synthesis, using a 2,3-ddA, 5-phosphoramidite. Purification must be by PAGE purification, since such an oligo will not have a trityl group (necessary for RPC purification).

ddC-3' should be used as a 3-end blocking moiety (see ddC technical sheet).

- dideoxy A 5'

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