dideoxy T 5'

Modification : ddT-5'

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6334
End Blockers
ddT-5'
Y
N
N
288.19
8.7
PS26-6334.pdf
-
-
-

Catalog No	Scale	Price
26-6334-05	50 nmol	$195.00
26-6334-02	200 nmol	$195.00
26-6334-01	1 umol	$292.00
26-6334-03	2 umol	$438.00
26-6334-10	10 umol	$2,336.00
26-6334-15	15 umol	$2,920.00

Dideoxythymidine (ddT) is a dideoxyribonucleoside that can only be used to block the 5' end. Use ddC-3' or Spacer C3 to block the 3' end from polymerase extension. ddT is a synthetic analog of deoxythymidine, in ddT, both the 2’- and 3’-positions of the ribose have a hydrogen (-H) group substituted for the –OH group, whereas in dT, only the 2’-position is so substituted. ddT is added to the 5’-end of an oligo via 5’-to-5’ synthesis, using a 2’,3’-ddT, 5’-phosphoramidite. Purification must be by PAGE purification, since such an oligo will not have a trityl group (necessary for RPC purification).

ddC-3' should be used as a 3’-end blocking moiety (see ddC technical sheet).

- dideoxy T 5'