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dideoxy T 5'

picture of dideoxy T 5'

Modification : ddT-5'

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6334
End Blockers
ddT-5'
Y
N
N
288.19
8.7
PS26-6334.pdf
-
-
-


Catalog NoScalePrice
26-6334-0550 nmol$195.00
26-6334-02200 nmol$195.00
26-6334-011 umol$292.00
26-6334-032 umol$438.00
26-6334-1010 umol$2,336.00
26-6334-1515 umol$2,920.00

Dideoxythymidine (ddT) is a dideoxyribonucleoside that can only be used to block the 5' end. Use ddC-3' or Spacer C3 to block the 3' end from polymerase extension. ddT is a synthetic analog of deoxythymidine, in ddT, both the 2- and 3-positions of the ribose have a hydrogen (-H) group substituted for the OH group, whereas in dT, only the 2-position is so substituted. ddT is added to the 5-end of an oligo via 5-to-5 synthesis, using a 2,3-ddT, 5-phosphoramidite. Purification must be by PAGE purification, since such an oligo will not have a trityl group (necessary for RPC purification).

ddC-3' should be used as a 3-end blocking moiety (see ddC technical sheet).

- dideoxy T 5'

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