In this section: Introduction | Quality Control | Purification | Modifications | Long Oligos | Price List
In this section: Introduction | Quality Control | Purification | Duplex Stability Mods | Modifications | Price List
In this section: Introduction | Molecular Beacon FAQ's | Fluorescent Probes Price List | Other Fluorescent Molecular Probes
In this section: SPCT | DME SPCT Intro | Order DME TaqMan® Assays SPCT | SNP PCT Search | Gene Expression Assays | SPCT Design Center | GeneAssays
In this section: RNA Oligonucleotides | Quality Control | Purification | Modifications | RNAi Explorer™ Products and Prices | Custom RNAi | RNAi Design Guidelines | SmartBase™ siRNA Modifications | shRNA Explorer™
In this section: PCR Amplification & Analysis
In this section: Introduction | Genemer™ | GeneProber™ | Prober™ Gene Detection Kits | GScan™ Gene Detection Kits | Genemer™ Control DNA | Infectious Diseases
In this section: Gene Construction
In this section: Introduction | The Omni-Clean™ System | The Omni-Pure™ Plasmid Purification System | The Omni-Pure™ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
In this section: Introduction | Quality Control | Purification | Modifications | Long Oligos | Price List
In this section: Introduction | Molecular Beacon FAQ's | Fluorescent Probes Price List | Other Fluorescent Molecular Probes
In this section: SPCT | DME SPCT Intro | Order DME TaqMan® Assays SPCT | SNP PCT Search | Gene Expression Assays | SPCT Design Center | GeneAssays
In this section: RNA Oligonucleotides | Quality Control | Purification | Modifications | RNAi Explorer™ Products and Prices | Custom RNAi | RNAi Design Guidelines | SmartBase™ siRNA Modifications | shRNA Explorer™
In this section: PCR Amplification & Analysis
In this section: Introduction | Genemer™ | GeneProber™ | Prober™ Gene Detection Kits | GScan™ Gene Detection Kits | Genemer™ Control DNA | Infectious Diseases
In this section: Gene Construction
In this section: Introduction | The Omni-Clean™ System | The Omni-Pure™ Plasmid Purification System | The Omni-Pure™ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
SmartBaseTM siRNA Yield & Price List
|
Crude Desalted |
RPC Purified |
Gel Purified |
Scale of Synthesis |
nmols |
mg |
A260 Units |
nmols |
mg |
A260 Units |
nmols |
mg |
A260 Units |
50 nmols |
~10 |
~0.06 |
~2 |
~5 |
~0.03 |
~1 |
~0.5 |
~0.003 |
~0.1 |
200 nmols |
~30 |
~0.2 |
~7 |
~15 |
~0.1 |
~3.5 |
~5 |
~0.03 |
~1 |
1 umol |
~150 |
~0.8 |
~25 |
~75 |
~0.4 |
~12 |
~25 |
~0.16 |
~5.5 |
10 umol |
~1.5 |
~8 |
~250 |
~600 |
~3.2 |
~110 |
~250 |
~1.16 |
~55 |
15 umol |
~2.25 |
~12 |
~375 |
~900 |
~5 |
~180 |
~375 |
~2.4 |
~82 |
Purity is greater than 75% depending on oligo sequence and structure. Reverse phase purification recommended for greater than 85% purity. Gel purification recommended to achieve purity above 95%.
|
Purity 85% to 95% depending on oligo sequence and structure. Yield and purity will be lower for sequences with high GC content
**RPC is reverse phase purification using a cartridge; a substitue for HPLC.
|
Purity +98% depending on oligo sequence and structure. Palindromes, hairpins and high GC content oligos and oligos containing stretches of 3 or more G's induce strong secondary structure and base stacking thus decreasing purity and yield.
|
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