SmartBase^TM siRNA

Crude Desalted

RPC Purified

Gel Purified

Scale of Synthesis

nmols

mg

A260 Units

nmols

mg

A260 Units

nmols

mg

A260 Units

50 nmols

~10

~0.06

~2

~5

~0.03

~1

~0.5

~0.003

~0.1

200 nmols

~30

~0.2

~7

~15

~0.1

~3.5

~5

~0.03

~1

1 umol

~150

~0.8

~25

~75

~0.4

~12

~25

~0.16

~5.5

10 umol

~1.5

~8

~250

~600

~3.2

~110

~250

~1.16

~55

15 umol

~2.25

~12

~375

~900

~5

~180

~375

~2.4

~82

Purity is greater than 75% depending on oligo sequence and structure. Reverse phase purification recommended for greater than 85% purity. Gel purification recommended to achieve purity above 95%.

Purity 85% to 95% depending on oligo sequence and structure. Yield and purity will be lower for sequences with high GC content **RPC is reverse phase purification using a cartridge; a substitue for HPLC.

Purity +98% depending on oligo sequence and structure. Palindromes, hairpins and high GC content oligos and oligos containing stretches of 3 or more G's induce strong secondary structure and base stacking thus decreasing purity and yield.