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RNAi Explorer
What is RNAi and siRNA? RNAi Explorer™ shRNA Explorer™ RNAi Explorer™ product line and price list. Guaranteed RNAi Explorer™ Kit

siRNA Synthesis

The siRNA duplexes provided are synthesized as a single strand oligo and annealed after complete deprotection and purification steps are completed. Gene Link utilizes the most reliable standard RNA synthesis chemistry. The RNA duplex is supplied lyophilized and ready to use.

 Handling & Storage

All siRNA are shipped lyophilized at room temperature. These are stable when kept lyophilized at room temperature. Follow established stringent RNase free handling conditions. The lyophilized siRNA duplex should be stored immediately at -20o C. The lyophilized siRNA is stable for ~6 months at -20o C.

Guaranteed RNAi Explorer™ Kit
Guaranteed RNAi Explorer Kit is only available for Human and Mouse Reference Sequence (RefSeq) Accession Numbers.
ComponentsQuantityPrice
3 Target siRNA’s10 nmol each$995
1 negative control siRNA5 nmol
1 QPCR probe or 6 free oligos* 5 nmol
*Select Choice when placing order.
27-6402-01 Guaranteed RNAi Explorer kit with Fluorescein/Dabcyl Molecular Beacon
27-6402-02 Guaranteed RNAi Explorer kit with Fluorescein/Tamra TaqMan
27-6402-06 Guaranteed RNAi Explorer kit with 6 oligos up to 30mer at 50 nmol scale
Click here to order.

siRNA (duplexed)
Catalog No.27-6401-0627-6401-0527-6401-0227-6401-10
Purification20 nmol scale*50 nmol scale*200 nmol scale*1 mmol scale*
Crude$195$295$420$650
RPC$225$325$450$740
Gel NA$370$495$800
*Please see table below for synthesis scale yields for crude, RPC and gel purified.
Click here to order.
siRNA Duplexed Scale of Synthesis and Typical Yield*

  Crude Desalted RPC Purified** Gel Purified
Scale nmols nmols nmols
20 nmol ~5 ~1 NA
50 nmol ~20 ~10 ~2
200 nmol ~75 ~40 ~25
1 umol ~200 ~160 ~100
Purity & Yield Purity is greater than 75%
depending on oligo sequence and structure. 

Reverse phase purification recommended.

Gel purification recommended to achieve purity above 95%.

Purity 85% to 95%
depending on oligo sequence and structure. Yield and purity will be lower for sequences with high GC content

**RPC is reverse phase purification using a cartridge; a substitue for HPLC.

 Purity 98% to ~100%
depending on oligo sequence and structure.

Palindromes, hairpins and high GC content oligos and oligos containing stretches of  3 or more G's induce strong secondary structure and base stacking thus decreasing purity and yield.

 

Control siRNA Duplexes*
ProductSizeCatalog NumberPrice,$
Negative Control, non-silencing unlabeled5 nmols27-6411-05$110.00
Negative Control, non-silencing unlabeled20 nmols27-6411-20$250.00
Negative Control, non-silencing Fl labeled2 nmols27-6411-02FL$110.00
Negative Control, non-silencing Fl labeled10 nmols27-6411-10FL$350.00
Negative Control, non-silencing Fl labeled20 nmols27-6411-20FL$495.00
*Please note catalog number change. Old Catalog Number was 27-6410-XXX
Positive Controls
Human Vimentin; NM_0033805 nmols27-6412-05$110.00
Human Vimentin; NM_00338020 nmols27-6412-20$250.00
Human Beta Actin; NM_0011015 nmols27-6413-05$110.00
Human Beta Actin; NM_00110120 nmols27-6413-20$250.00
Mouse Beta Tubulin; AF3128735 nmols27-6414-05$110.00
Mouse Beta Tubulin; AF31287320 nmols27-6414-20$250.00
Rat Chromogranin-A; NM_0216555 nmols27-6415-05$110.00
Rat Chromogranin-A; NM_02165520 nmols27-6415-20$250.00
Fluorescein Labeled Controls
Human Vimentin; NM_003380; FL labeled2 nmols27-6412-02FL$110.00
Human Vimentin; NM_003380; FL labeled10 nmols27-6412-10FL$350.00
Human Beta Actin; NM_001101; FL labeled2 nmols27-6413-02FL$110.00
Human Beta Actin; NM_001101; FL labeled10 nmols27-6413-10FL$350.00
Mouse Beta Tubulin; AF312873; FL labeled2 nmols27-6414-02FL$110.00
Mouse Beta Tubulin; AF312873; FL labeled10 nmols27-6414-10FL$350.00
Rat Chromogranin-A; NM_021655;FL labeled2 nmols27-6415-02FL$110.00
Rat Chromogranin-A; NM_021655;FL labeled10 nmols27-6415-10FL$350.00
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shRNA (DNA oligonucleotides)
Product200 nmol scale1 mmol scale
DNA Oligo Synthesis$1.80/base$3.25/base
Gel Purification$75.00$150.00
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*RNAi and siRNA
RNA interference (RNAi) is a specific and sequence dependent targeted gene silencing activity. RNAi acts by post transcriptional degradation of mRNA by small interfering RNAs (siRNA’s) of the same sequence. The silencing approaches 100% and has to be empirically determined and optimized. Not every siRNA can effectively down regulate a gene. The process of RNA interference varies by individual siRNA while some do not exhibit any interference at all.