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Gene Link shRNA Design Guidelines

GeneID:

Accession Number:

Upload Sequence from a file:

   

Type in or Copy/Paste Sequence
Sequence Name:

Start withNN
AA
CA, GA or TA
GC Content
30%-40%
40%-50%
50%-60%
Other Options
ExcludeGGG
ExcludeCCC
ExcludeAAA
ExcludeTTT

shRNA construct options

Loop Options
TTCG
TCAAGAG
GAAGCTTG
other
RNA Length
23 mer
29 mer
other
Foward Oligo cloning site
5' sequence 
3' sequence 
Reverse Oligo cloning site
5' sequence 
3' sequence 
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