Click here for a list of fluorophores.
VIC is a fluorescent dye that was originally developed by Applied Biosystems. VIC is available for 5' and also as NHS for internal sites with an amino group base or internal non-nucleosidic amino group. VIC has an excitation maximum of 538 nm and an emission maximum of 554 nm, thus emitting in the green-yellow part of the visible spectrum. It is used to fluorescently label oligonucleotides at the 5' end (for use as probes in a variety of real-time PCR, hybridization, and fluorescence-based genetic analysis applications) and is currently only available from Life Technologies. Since the fluorescent dye HEX has a similar emission profile (absorbance maximum = 535 nm; emission maximum = 556 nm), for those customers who are able, Gene Link offers HEX as a less expensive reasonable substitute. For more information on HEX, please see its technical sheet.
|
Applied Biosystems Proprietary Dyes & Possible Substitutions
|
Dye |
Color |
Absorbance max (nm) |
Emission max (nm) |
| VIC |
Pink Red |
538 |
554 |
| Cal Orange 560 |
Pink Red |
537 |
558 |
| HEX |
Pink Red |
535 |
556 |
|
|
| NED |
Red Orange |
546 |
575 |
| Cy3 |
Red Orange |
550 |
570 |
|
|
| PET |
Red Orange |
558 |
595 |
| Cy3.5 |
Red |
588 |
604 |
| ROX |
Red |
575 |
602 |
| Texas Red |
Red |
583 |
603 |
Click here for a list of fluorophores.
- VIC 5' fluorophore | 
Oligo Purification