Amino dT C6 can be used to internally incorporate an active primary amino group into either a DNA oligonucleotide or a chimeric oligo. The presence of the primary amino group allows the user to label the oligo with a variety of different ligands for affinity, reporter or protein moieties (as NHS esters or isothiocyanates), depending on the application. Examples include biotin, digoxigenin, and fluorescent dyes or quenchers, magnetic beads and enzymes (for example, alkaline phosphatase).
NHS ester-activated ligands react with primary amines to yield stable amide bonds. The reaction releases N-hydroxysuccinimide (NHS). NHS ester reaction scheme for chemical conjugation to a primary amine in an oligo is given below.
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The primary amine labelled oligos can also be conjugated to carboxyl functional groups usually for solid supports applications using EDC mediated reaction as shown in the figure below.
- Amino deoxythymidine dT C6 | 
Oligo Purification