RNA and 2’-O-methyl RNA oligonucleotide synthesis is performed at Gene Link using the b –cyanoethyl chemistry and state of the art synthesizers. These involve proprietary software protocols with long coupling times and specialized cycles to obtain ultra clean oligos.
RNA oligos are susceptible to degradation to the same extent as native RNA extracted from various sources. An attractive alternate to prevent degradation from nucleases is the use of 2’-O- methyl RNA bases, when specific 2’OH is not required. The 2’-O- methyl oligonucleotides confer considerable nuclease resistance and are similar in hydrogen bonding properties to RNA/RNA than the lower RNA/DNA binding property. The coupling efficiency of 2’-O- methyl phosphoramidite is also higher than the RNA monomers resulting in higher yield of full length oligos.
Gene Link also offers custom synthesis of RNA and DNA chimeric oligos with investigator specified ribo or deoxy bases or 2’-O-methyl bases. The chimeric oligos can also be synthesized with the regular phoshodiester bonds or substituted with phosphorothioate linkages. The combination of 2’-O- methyl RNA bases with phosphorothioate internucleotide linkages imparts these oligos greater nuclease resistance which is particularly useful for antisense studies (please refer to our technical sheet on Antisense Oligonucleotides for other modifications). Custom phosphorothioate oligonucleotides synthesized by Gene Link can be specified to have all the diester bonds substituted or only some selected diester linkages depending upon the researchers experimental requirement. Substitution of all diester linkage is recommended to provide greater nuclease resistance.
What is RNAi and siRNA?
RNAi Explorer™ product line and price list.
Guaranteed RNAi Explorer™ Kit*
3+1 siRNA’s and a detection probe. You simply give us the accession number or sequence for the gene to down regulate. Gene Link designs siRNA’s using published criteria’s and proprietary algorithms. Gene Link guarantees a minimum of 70% silencing of your gene with at least one of the siRNA supplied. Three target and a negative control siRNA will be supplied and a detection probe of your choice. All for $995
Guaranteed RNAi Explorer Kit is
only available for Human and Mouse Reference Sequence (RefSeq) Accession Numbers.
RNAi Explorer™
Custom siRNA search and design. Featuring Gene Link’s proprietary algorithms to design siRNA’s bound to silence the gene.
Oligos for shRNA constructs
Designing expression vectors for your shRNA?
RNAi - Technical Information