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Inosine ribo

picture of Inosine ribo

Modification : Inosine ribo

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



27-6421
Others
rI
Y
Y
Y
330.19
7.5
PS27-6421.pdf
-
-
-


Catalog NoScalePrice
27-6421-0550 nmol$84.00
27-6421-02200 nmol$84.00
27-6421-011 umol$109.20
27-6421-032 umol$163.80
27-6421-1010 umol$873.60
27-6421-1515 umol$1,092.00
Discounts are available for Inosine ribo!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply
Related Modifications
Inosine deoxy
2'-O methyl Inosine

Inosine (dI & rI) is classified as a nucleotide base analog; it is structurally similar to guanosine, but is missing the 2-amino group. Because it is able to form two hydrogen bonds with each of the four natural nucleotide bases (1), it is often used by researchers as a universal basemeaning that it can base pair with all the naturally-occurring bases--in synthetic oligos. Inosine typically is substituted for the nucleoside at the third (wobble) position of codons, in order to reduce the complexity of mixed oligo PCR primers/hybridization probes needed to deal with degenerate codons in the target DNA (2, 3). However, it is important to remember that I does not base pair equally well with the naturally-occurring bases, with the order of thermodynamic stability being I-C >: I-A > I-G ~ I-T. Thermodynamic stability of inosine-containing duplexes is also affected by neighboring bases (4). Consequently, when using inosine as an alternative to mixed-base degeneracy at a particular oligo position, keep in mind that the above base-pairing bias may lead to differences in the oligos priming or hybridization efficiency in the corresponding degenerate regions of the target. Because the effect could be more pronounced when dI is at the 3-position, it may be advisable to use primers with and without I at the 3-end, in order to maximize diversity of PCR products (5).

References
1. Oda, Y, Uesugi, S., Ikehara, M., Kawase, Y., Ohtsuka, E. NMR studies for identification of dI:dG mismatch base-pairing structure in DNA. Nucleic Acids Res. (1991), 19: 5263-5267.
2. Liu, H., Nichols, R. PCR amplification using deoxyinosine to replace entire codon and at ambiguous positions.Biotechniques. (1994), 16: 24-26.
3. Ohtsuka, E., Matsuki, S., Ikehara, M., Takahashi, Y., Matsubara, K. An alternative approach to deoxynucleotides as hybridization probes by insertion of deoxyinosine at ambiguous codon positions. J. Biol. Chem. (1985), 260: 2605-2608.
4. Martin, F.H., Castro, M.M., Aboul-ela, F., Tinoco, I. Base pairing involving deoxyinosine: implications for probe design. Nucleic Acids Res. (1985), 13: 8927-8938.
5. Ben-Dov, E., Shapiro, O.H., Siboni, N., Kushmaro, A. Advantage of Using Inosine at the 3 Termini of 16S rRNA Gene Universal Primers for the Study of Microbial Diversity. Appl. Environ. Microb. (2006), 72: 6902-6906.
- Inosine ribo

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