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rSpacer (ribo Spacer)

picture of  rSpacer (ribo Spacer)

Modification : rSpacer

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6442
Spacers
rSpc
Y
Y
Y
196.09
-
PS26-6442-02.pdf
-
-
-


Catalog NoScalePrice
26-6442-0550 nmol$180.00
26-6442-02200 nmol$180.00
26-6442-011 umol$234.00
26-6442-032 umol$351.00
26-6442-1010 umol$1,872.00
26-6442-1515 umol$2,340.00
Discounts are available for rSpacer!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

RiboSpacer (rSpacer) is a tetrahydrofuran derivative, in which a methylene group occupies the 1 position of 2-ribose. rSpacer is commonly used to mimic an abasic site in an RNA oligonucleotide. Naturally-occurring abasic sites in RNA are less common than in DNA, due to RNA being less susceptible to depurination (1). However, once generated, either spontaneously or via an enzymatic pathway, RNA abasic sites are about 15-fold more stable than DNA abasic sites; this fairly high level of stability could have important biological consequences for long-lived RNAs (for example, tRNAs or rRNA) (2). While such biological consequences have been largely unexplored thus far, abasic site effects on RNA structure and activity has been observed for the case of the hammerhead ribozyme, which catalyzes phosphodiester bond cleavage (3). Introduction of abasic sites at different positions of this ribozymes core significantly reduced ribozyme activity. Interestingly, the activity was partially rescued for some abasic positions by exogenous addition of the missing base. rSpacer-modified oligonucleotides could serve as important research tools for elucidating the effects of abasic sites on the structure and function of long-lived RNAs and ribozymes.

References
1. Kochetkov, N.K., Budovskii, E.I. Hydrolysis of N-glycosidic bonds in nucleosides, nucleotides and their derivatives. In Organic Chemistry of Nucleic Acids New York: Plenum; (1993). pp. 425-448.
2. Kupfer, P.A., Leumann, C.J. The chemical stability of abasic RNA compared to abasic DNA. Nucleic Acids Res. (2007), 35: 58-68.
3. Peracchi, A., Beigelman, L., Usman, N., Herschlag, D. Rescue of abasic hammerhead ribozymes by exogenous addition of specific bases. Proc. Natl. Acad. Sci. USA. (1996), 93: 11522-11527.
- rSpacer (ribo Spacer)

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