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PC Spacer (photocleavable)

picture of PC Spacer (photocleavable)

Modification : PC Spacer (photocleavable)

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6889
Photo Cleavable
SpPC
Y
N
Y
344.26
-
PS26-6889.pdf
-
-
-


Catalog NoScalePrice
26-6889-0550 nmol$170.00
26-6889-02200 nmol$170.00
26-6889-011 umol$210.00
26-6889-032 umol$315.00
26-6889-1010 umol$1,680.00
26-6889-1515 umol$2,100.00
Discounts are available for PC Spacer (photocleavable)!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

PC Spacer (photocleavable) is a non-nucleosidic molety that can be used as an intermediary to attach any available phosphoramidite modification at either end of an oligonucleotide through a UV photo-cleavable C3 spacer, as well as insert such a spacer internally. An example is the use of PC Spacer to incorporate a photo-cleavable 6-FAM tag onto the 5-end of oligonucleotides immobilized on glass slides. These fluorescently-labeled oligo arrays were then UV irradiated in order to test the efficacy of photo-cleavage in removing the 6-FAM tag from these oligos, as part of developing sequencing-by-synthesis applications (1).

Cleavage Protocol


Cleavage occurs by irradiation with near-UV light (300-350 nm, complete cleavage occurs within 5 minutes. Try using a Black Ray XX-15 UV lamp (Ultraviolet Products Inc., San Gabriel, CA) at a distance of 15 cm (emission peak 365 nm, 300 nm cut-off, 1.1 mW intensity at~31 cm).

References


1. Olejnik, J., Krzymanska-Olejnik, E., Rothschild, K.J. Photocleavable aminotag phosphoramidites for 5-termini DNA/RNA labeling. Nucleic Acids Res. (1998), 26: 3572-3576.
2. Olejnik, J., Ludemann, H-C., Olejnik, E.K, Berkenkamp, S., Hillenkamp, F., Rothschild, K.J. Photocleavable peptide-DNA conjugates: synthesis and applications to DNA analysis using MALDI-MS. Nucleic Acids Res. (1999), 27: 4626-4631.
3. Tang, X., Su, M., Yu, LiLi, Lv, C., Wang, J., Li, Z. Photomodulating RNA cleavage using photolabile circular antisense oligodeoxynucleotides.Nucleic Acids Res. (2002), 38: 3848-3855.
- PC Spacer (photocleavable)

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