TaqMan - Molecular Beacons - Fluorescent Molecular Probes

TaqMan probes and Molecular beacons with universal fluorescence quencher dabcyl or Tamra or BHQ at 3' end. Gene Link considers gel purification (PAGE) to be the gold standard method of purification and essential for optimum performance of fluorescent dye labeled oligonucleotides.Customers may request Molecular Beacons without gel purification for reduced pricing.

Special Pricing Fluorescent Probes
Price includes reverse phase purification. Guaranteed yield for dye and quencher listed.
Probe Type & Dye/Quencher	Price	Yield
5' 6-FAM/3' BHQ-1	$120.00	5 nmol
Hex, Tet, Cy3 or Cy5/3' BHQ-1 or BHQ-2	$230.00	5 nmol
Price includes synthesis and reverse phase purification of DNA oligo up to 32mer. Additional per base cost above 32mer. Price subject to change without notice.

Fluorescent Probes Price List Summary* and PAGE Purified Yield**
	50 nmol scale		200 nmol scale		1 umol scale
Dye Quencher	Price	Yield	Price	Yield	Price	Yield
5' 6-FAM and 3'-BHQ-1 or Tamra or Dabcyl	$190.00	10 nmol	$250.00	25 nmol	$350.00	60 nmol
See above for special pricing for 5 nmol final RPC purified yield probes.
5'-Hex, Tet, Cy 3 or Cy 5/Quencher	$230.00	5 nmol	$290.00	15 nmol	$450.00	40 nmol
Cy3.5, Cy5.5/Quencher	$390.00	5 nmol	$440.00	12 nmol	$650.00	25 nmol
Cy7-NHS, 5'-NHS Dyes (Rox, AZDye, other)/Quencher	$410.00	2 nmol	$490.00	5 nmol	$750.00	16 nmol
Other NHS Fluorophores/Quencher	$490.00	2 nmol	$510.00	5 nmol	$790.00	16 nmol
*Price includes gel purification and does not include per base oligo synthesis charges. Royalty charges are additional. Pricing subject to change without notice.
**Approximate yield for dye and quencher listed. Lower yield with additional modifications.

Click here for a list of fluorophores.

Popular Fluorphores & Quenchers

Quencher & Fluorophore	Color	Suitable Quencher	Absorbance max (nm) ±10	Emission max (nm) ±10
Dabcyl (Quencher)			453 (380-530)
BHQ-1 (Quencher)			534 (480-580)
BHQ- 2 (Quencher)			579 (550-650)
BHQ-3 (Quencher)			672 (620-730)
BBQ-650 (Quencher)			650 (550-750)
6-FAM (Fluorescein)	Green	BHQ-1	494	525
TET	Orange	BHQ-1	521	536
JOE	Mustard	BHQ-1	528	554
HEX	Pink	BHQ-1	535	556
Cy 3	Red	BHQ-2	552	570
TAMRA	Rose	BHQ-2	565	580
Cy 3.5	Purple	BHQ-2	588	604
ROX	Purple	BHQ-2	587	607
Cy 5	Violet	BBQ-650	646	667
Cy 5.5	Blue	BBQ-650	683	707
Cy 7	Near IR	BBQ-650	743	767
More information on fluorescent dyes is available at this link. Fluorescent Dyes Reference & Price List

**Black Hole Quencher License Agreement
"Black Hole Quencher", "BHQ-1", "BHQ-2" and "BHQ-3" are registered trademarks of Biosearch Technologies, Inc., Novato, CA. The BHQ technology is licensed and sold under agreement with Biosearch and these products are sold exclusively for R&D use by the purchaser. They may not be used for clinical or diagnostic purposes and they may not be resold, distributed or re-packaged

Fluorophore Spectral Data & Quencher Selection Guide

Fluorophore Name	Excitation Max, nm ±10	Emission Max, nm ±10	Extinction Coefficient*	Color**	Quencher
AZDye-350 NHS	346	445	19,000	Blue	Dabcyl λ (max) = 453 nm Range = 380-530 nm
AZDye-405 NHS	402	424	33,000	Blue	Dabcyl λ (max) = 453 nm Range = 380-530 nm
PBlue-455 NHS	416	451	46,000	Blue	Dabcyl λ (max) = 453 nm Range = 380-530 nm
MBlue-460 NHS	362	459	20,000	Blue-Green	Dabcyl λ (max) = 453 nm Range = 380-530 nm
AZDye-488 NHS	494	517	73,000	Yellow-Green	BHQ-1 λ (max) = 534 nm Range = 480-580 nm
FAM	495	520	75,850	Yellow-Green	BHQ-1 λ (max) = 534 nm Range = 480-580 nm
TET	521	536	99,000	Yellow-Green	BHQ-1 λ (max) = 534 nm Range = 480-580 nm
AZDye-430 NHS	430	539	15,000	Yellow-Green	BHQ-1 λ (max) = 534 nm Range = 480-580 nm
Cal Fluor Gold 540	552	543	81,100	Yellow	BHQ-1 λ (max) = 534 nm Range = 480-580 nm
JOE	520	548	75,000	Yellow	BHQ-1 λ (max) = 534 nm Range = 480-580 nm
Yakima Yellow	531	549	83,800	Yellow	BHQ-1 λ (max) = 534 nm Range = 480-580 nm
AZDye-532 NHS	530	555	81,000	Yellow	BHQ-1 λ (max) = 534 nm Range = 480-580 nm
HEX	535	556	98,000	Yellow	BHQ-1 λ (max) = 534 nm Range = 480-580 nm
Cal Orange 560	537	558	150,000	Yellow	BHQ-2 λ (max) = 579 nm Range = 550-650 nm
CY3	550	570	150,000	Yellow-Orange	BHQ-2 λ (max) = 579 nm Range = 550-650 nm
AZDye-555 NHS	555	572	155,000	Yellow-Orange	BHQ-2 λ (max) = 579 nm Range = 550-650 nm
TAMRA	555	576	65,000	Yellow-Orange	BHQ-2 λ (max) = 579 nm Range = 550-650 nm
CAL Fluor Red 590	569	591	79,000	Yellow-Orange	BHQ-2 λ (max) = 579 nm Range = 550-650 nm
Redmond Red	579	595	52,300	Yellow-Orange	BHQ-2 λ (max) = 579 nm Range = 550-650 nm
CY3.5	581	596	150,000	Yellow-Orange	BHQ-2 λ (max) = 579 nm Range = 550-650 nm
ROX NHS	575	602	82,000	Orange	BHQ-2 λ (max) = 579 nm Range = 550-650 nm
AZDye-568 NHS	578	602	88,000	Orange	BHQ-2 λ (max) = 579 nm Range = 550-650 nm
Cal Red 610	590	610	108,000	Orange	BHQ-2 λ (max) = 579 nm Range = 550-650 nm
TXRed-616 NHS	595	615	80,000	Orange	BHQ-2 λ (max) = 579 nm Range = 550-650 nm
AZDye-594 NHS	590	617	92,000	Orange-Red	BHQ-2 λ (max) = 579 nm Range = 550-650 nm
CAL Fluor Red 635	616	637	112,000	Red	BBQ-650 λ (max) = 650nm Range = 550-750 nm
LC Red 640 NHS	625	640	110,000	Red	BBQ-650 λ (max) = 650nm Range = 550-750 nm
AZDye-647 NHS	649	671	270,000	Red	BBQ-650 λ (max) = 650nm Range = 550-750 nm
Cy5	649	670	250,000	Red	BBQ-650 λ (max) = 650nm Range = 550-750 nm
Cy5.5	675	694	190,000	Red	BBQ-650 λ (max) = 650nm Range = 550-750 nm
AZDye-680 NHS	678	701	185,000	Near-IR region. Human vision is insensitive to light beyond ~650 nm; it is not possible to view near-IR fluorescent dyes.	BBQ-650 λ (max) = 650nm Range = 550-750 nm
Cy7 NHS	750	773	199,000	Near-IR region. Human vision is insensitive to light beyond ~650 nm; it is not possible to view near-IR fluorescent dyes.	BBQ-650 λ (max) = 650nm Range = 550-750 nm
IR 750 NHS	756	776	260,000	Near-IR region. Human vision is insensitive to light beyond ~650 nm; it is not possible to view near-IR fluorescent dyes.	BBQ-650 λ (max) = 650nm Range = 550-750 nm
Cy7.5 NHS	788	808	223,000	Near-IR region. Human vision is insensitive to light beyond ~650 nm; it is not possible to view near-IR fluorescent dyes.	BBQ-650 λ (max) = 650nm Range = 550-750 nm
* Extinction coefficient at λ (max) in cm–1M–1. ** Typical emission color seen through the eyepiece of a conventional fluorescence microscope with appropriate filters.