In this section: Introduction | Quality Control | Purification | Modifications | Long Oligos | Price List
In this section: Introduction | Molecular Beacon FAQ's | Fluorescent Probes Price List | Other Fluorescent Molecular Probes
In this section: SPCT | DME SPCT Intro | Order DME TaqMan® Assays SPCT | SNP PCT Search | Gene Expression Assays | SPCT Design Center | GeneAssays
In this section: RNA Oligonucleotides | Quality Control | Purification | Modifications | RNAi Explorer™ Products and Prices | Custom RNAi | RNAi Design Guidelines | SmartBase™ siRNA Modifications | shRNA Explorer™
In this section: PCR Amplification & Analysis
In this section: Introduction | Genemer™ | GeneProber™ | Prober™ Gene Detection Kits | GScan™ Gene Detection Kits | Genemer™ Control DNA | Infectious Diseases
In this section: Gene Construction
In this section: Introduction | The Omni-Clean™ System | The Omni-Pure™ Plasmid Purification System | The Omni-Pure™ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
In this section: Introduction | Quality Control | Purification | Modifications | Long Oligos | Price List
In this section: Introduction | Molecular Beacon FAQ's | Fluorescent Probes Price List | Other Fluorescent Molecular Probes
In this section: SPCT | DME SPCT Intro | Order DME TaqMan® Assays SPCT | SNP PCT Search | Gene Expression Assays | SPCT Design Center | GeneAssays
In this section: RNA Oligonucleotides | Quality Control | Purification | Modifications | RNAi Explorer™ Products and Prices | Custom RNAi | RNAi Design Guidelines | SmartBase™ siRNA Modifications | shRNA Explorer™
In this section: PCR Amplification & Analysis
In this section: Introduction | Genemer™ | GeneProber™ | Prober™ Gene Detection Kits | GScan™ Gene Detection Kits | Genemer™ Control DNA | Infectious Diseases
In this section: Gene Construction
In this section: Introduction | The Omni-Clean™ System | The Omni-Pure™ Plasmid Purification System | The Omni-Pure™ Genomic DNA Purification System | Viral DNA & RNA Purification | Microbial DNA Purification | Plant DNA Purification
PBlue NHS
Modification : PBlue NHS
Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC
26-6524
Fluorescent Dyes
[PB-N]
Y
Y
Y
339.21
30
PS26-6524.pdf
410
455
30
Catalog No | Scale | Price | 26-6524-05 | 50 nmol | $350.00 | 26-6524-02 | 200 nmol | $350.00 | 26-6524-01 | 1 umol | $455.00 | 26-6524-03 | 2 umol | $682.50 | 26-6524-10 | 10 umol | $3,640.00 | 26-6524-15 | 15 umol | $4,550.00 |
PBlue fluorescent dye modification is a post synthesis conjugation to a primary amino group. The amino group can be placed at the 5' and 3' and for internal positions an amino modified base is used, e.g Amino dT C6
PBlue is a UV-excitable, bright blue fluorescent dye used for labeling oligonucleotides excitable by the 407 nm spectral line of blue diode (violet color) laser. PBlue has an absorbance maximum of 401 nm and an emission maximum of 421 nm. Because UV light can photo damage labeled oligos, and many kinds of cells and tissues autofluoresce under UV light, PBlue can only be used in a limited number of applications. Nevertheless, for such applications as nucleic acid microarrays and in situ hybridization, where a blue fluorescent probe provides a easily distinguishable, contrasting color to the green, yellow, orange and red fluorescence produced by longer-wavelength probes, PBlue can be a good choice. - PBlue NHS
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