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PBlue NHS

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Modification : PBlue NHS

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6524
Fluorescent Dyes
[PB-N]
Y
Y
Y
339.21
30
PS26-6524.pdf
410
455
30


Catalog NoScalePrice
26-6524-0550 nmol$350.00
26-6524-02200 nmol$350.00
26-6524-011 umol$455.00
26-6524-032 umol$682.50
26-6524-1010 umol$3,640.00
26-6524-1515 umol$4,550.00

PBlue fluorescent dye modification is a post synthesis conjugation to a primary amino group. The amino group can be placed at the 5' and 3' and for internal positions an amino modified base is used, e.g Amino dT C6

PBlue is a UV-excitable, bright blue fluorescent dye used for labeling oligonucleotides excitable by the 407 nm spectral line of blue diode (violet color) laser. PBlue has an absorbance maximum of 401 nm and an emission maximum of 421 nm. Because UV light can photo damage labeled oligos, and many kinds of cells and tissues autofluoresce under UV light, PBlue can only be used in a limited number of applications. Nevertheless, for such applications as nucleic acid microarrays and in situ hybridization, where a blue fluorescent probe provides a easily distinguishable, contrasting color to the green, yellow, orange and red fluorescence produced by longer-wavelength probes, PBlue can be a good choice.

- PBlue NHS

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