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Cy5 disulfo NHS

Cy5 disulfo NHS

Code : [Cy5-S2-N]

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picture of Cy5 disulfo NHS

Modification : Cy5 disulfo NHS

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6443
Fluorescent Dyes
[Cy5-S2-N]
Y
Y
Y
533.63
-
26-6443.pdf
646
667
271000


Catalog NoScalePrice
26-6443-0550 nmol$267.00
26-6443-02200 nmol$267.00
26-6443-011 umol$362.00
26-6443-032 umol$543.00
26-6443-1010 umol$2,894.00
26-6443-1515 umol$3,618.00

Cy5 disulfo NHS modification is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is required. A C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

The diSulfo Cy5 NHS Ester is a hydrophilic version of Cy5 due to the two sulfo groups. This version is particularly helpful when the standard hydrophobic Cy5 version is not appropriate for the desired application. All NHS ester derivative modifications are post synthesis for oligos and requires a primary amino group on the oligo for conjugation. The amino group can be placed at either the 5 or 3 ends an internally as well.
Cy5 can be used as a replacement for Alexa Fluor 647 Succinimidyl Ester, DyLight 650 NHS Ester, Colorada 645 XT A - NHS ester, Fluorescentred 647 reactive, CF647 uccinimidyl ester and PromoFluor -647, NHS ester for the required applications.

Near Infrared Fluorophore Spectral Data & Quencher Selection Guide

Fluorophore Name

Excitation Max, nm +/-10

Emission Max, nm +/-10

Extinction Coefficient*

Color**

Quencher

Cy5

650 665 250,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

IRDye 650 NHS

650 665 230,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

AZ647 NHS

655 680 191,800

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

Cy5.5

684 710 198,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

IRDye 700 NHS

684 710 288,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

Cy7 NHS

740 773 199,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

IRDye 750 NHS

756 776 260,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

cy7.5 NHS

788 808 223,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

IRDye 800 NHS

795 819 240,000

Human vision is insensitive to light beyond ~650 nm.

BBQ-650 λ (max) = 650nm Range = 550-750 nm

* Extinction coefficient at λ (max) in cm-1M-1. ** Typical emission color seen through the eyepiece of a conventional fluorescence microscope with appropriate filters. Near-IR region. Human vision is insensitive to light beyond ~650 nm; it is not possible to view near-IR fluorescent dyes.

Click here for a list of fluorophores.

Click here for list of quenchers.



References
1. Livak, K.J., Flood, S.J.A., Marmaro, J., Giusti, W., Deetz, K. Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization.PCR Methods Appl. (1995), 4: 1-6.
2. Thelwell, N., Millington, S., Solinas, A., Booth, J., Brown, T. Mode of action and application of Scorpion primers to mutation detection. Nucleic Acids Res. (2000), 28: 3752-3761.
3. Tyagi, S., Kramer, F.R. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. (1996), 14: 303-308.
4. Stoecker, K., Dorninger, C., Daims, H., Wagner, M. Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH) Improves Signal Intensity and Increases rRNA Accessibility. Appl. Environ. Microb.. (2010), 76: 922-926.

Reaction scheme for primary amine labelled oligos with NHS ester is shown in the figure below.


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- Cy5 disulfo NHS

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