Cy2 (Cyanine 2)-NHS

Cy2 N

Code : [Cy2-N]

Modification : Cy2 N

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-7040
Fluorescent Dyes
[Cy2-N]
Y
Y
Y
897
15
PS26-6940.pdf
492
510
15

picture of Cy2 (Cyanine 2)-NHS

Catalog No	Scale	Price
26-7040-05	50 nmol	$1,512.00
26-7040-02	200 nmol	$1,512.00
26-7040-01	1 umol	$1,966.00
26-7040-03	2 umol	$2,948.00
26-7040-06	5 umol	$8,847.00
26-7040-10	10 umol	$15,725.00
26-7040-15	15 umol	$19,656.00

Related Modifications
Cy5 disulfo NHS	Cy3-5'	Cy7 NHS
Cy3 NHS	Cy3.5-5'	Cy3B-N
Cy5-5'	Cy5 NHS

Click here for a list of fluorophores.

This modification is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is required. A C3, C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

Yield of Post Synthesis NHS, Maleimide & Click Ligand Conjugation*
Oligo Scale of Synthesis	Yield, nmols
50 nmol	2 nmol
200 nmol	5 nmol
1 umol	16 nmol
2 umol	30 nmol
5 umol	75 nmol
10 umol	150 nmol
15 umol	225 nmol
* The yield will be lower for oligos longer than 50mer. Click here for yield table of long oligos.
* Click here for RNA Oligos scale of synthesis and yield.
NHS Ligand conjugation requires a primary amino group. Gene Link offers a wide selection of amino modifications for 5', 3' and internal sites. Click here for a list of conjugation chemistry modifications.
Maleimide Ligand conjugation requires a thiol group. Gene Link offers a wide selection of thiol modifications for 5', 3' and internal sites. Click here for a list of conjugation chemistry modifications.
Click Chemistry Ligand conjugation requires a corresponding Click modification; examples Alkyne:Azide, Azide:DBCO, BCN:Azide, BCN: TCO:Tetrazine. Gene Link offers a wide selection of click modifications for 5', 3' and internal sites. Click here for a list of click chemistry modifications.

Cy2 is a bright fluorescent dye that emits in the visible spectrum and can be used to label oligonucleotides. Cy2 has an absorbance maximum of 492 nm and an emission maximum of 510 nm. Cy2 is most commonly used to label the internal standard when such is required in an experiment (for example, difference gel electrophoresis (DIGE).

- Cy2 (Cyanine 2)-NHS