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DBCO-C6 sulfo NHS

DBCO-C6 NHS

Code : [DBCO-C6-N]

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picture of DBCO-C6 sulfo NHS

Modification : DBCO-C6 NHS

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC



26-6929
Click Chemistry
[DBCO-C6-N]
Y
Y
Y
316.37
-
PS26-6929.pdf
-
-
-


Catalog NoScalePrice
26-6929-0550 nmol$308.00
26-6929-02200 nmol$308.00
26-6929-011 umol$394.00
26-6929-032 umol$513.00
26-6929-1010 umol$1,348.00
26-6929-1515 umol$1,685.00

This modification is a post synthesis NHS conjugation to a primary amino group thus an additional modification with an amino group is required. A C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

YIELD
NHS based modifications are post synthesis conjugation performed using a primary amino group. The yield is lower as compared to direct automated coupling of modifications that are available as amidites. Approximate yield for various scales are given below.

~2 nmol final yield for 50 nmol scale synthesis.
~5 nmol final yield for 200 nmol scale synthesis.
~16 nmol final yield for 1 umol scale synthesis
~160 nmol final yield for 10 umol scale synthesis
~240 nmol final yield for 15 umol scale synthesis

Click here for a complete list of Click Chemistry Oligo Modifications



Cyclooctyne-based (dibenzocyclooctynes, DBCO) modifications offers the ease of copper-free click reagents. These are simple to use and has excellenet click performance in 17 hours or less at room temperature. Gene Link offers 5'-DBCO-TEG for preparing oligos with 5-DBCO and a 15 tom triethylene glycol spacer arm, DBCO-dT for inserting a DBCO group at any position within the oligonucleotide and DBCO-sulfo-NHS Ester is also offered for post-synthesis conjugation reactions. DBCO-modified oligos may be conjugated with azides in organic solvents, such as DMSO, or aqeous buffers. Depending on the azide used, the reaction will go to completion in 4-17 hours at room temperature.


Addition of DBCO-Sulfo-NHS is post synthesis and requires synthesis of oligo with primary amino group.



- DBCO-C6 sulfo NHS

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