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Amino Mod C6

Amino C6

Code : [AmC6]

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Modification : Amino C6

Catalog Reference Number
Modification Code
5 Prime
3 Prime
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

Conjugation Chemistry

Catalog NoScalePrice
26-6418-0550 nmol$85.00
26-6418-02200 nmol$85.00
26-6418-011 umol$119.00
26-6418-032 umol$142.00
26-6418-065 umol$319.50
26-6418-1010 umol$714.00
26-6418-1515 umol$856.00
Discounts are available for Amino C6!
Modification* Discount Price Structure
1 site/order List price
2 sites/order 10% discount
3 sites/order 20% discount
4 sites/order 30% discount
5-9 sites/order 50% discount
10+ sites/order 60% discount
*Exceptions apply

Amino Linker C6 can be used to incorporate an active primary amino group onto the 5'-end of an oligonucleotide. This can then be conjugated to a NHS Activated ligand. The amino group then becomes internal to the 5' end ligand. The amino group is separated from the 5'-end nucleotide base by a 6-carbon spacer arm to reduce steric interaction between the amino group and the oligo.
The presence of the primary amino group allows the user to label the oligo with a variety of different ligands for affinity, reporter or protein moieties (as NHS esters or isothiocyanates), depending on the application. Examples include biotin, digoxigenin, and fluorescent dyes or quenchers, magnetic beads and enzymes (for example, alkaline phosphatase).

NHS ester-activated ligands react with primary amines to yield stable amide bonds. The reaction releases N-hydroxysuccinimide (NHS). NHS ester reaction scheme for chemical conjugation to a primary amine in an oligo is given below.


The primary amine labelled oligos can also be conjugated to carboxyl functional groups usually for solid supports applications using EDC mediated reaction as shown in the figure below.

- Amino Mod C6

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