PBlue-455 NHS

Code : [PBlue-455]

Modification : PBlue-455 NHS

Catalog Reference Number
Category
Modification Code
5 Prime
3 Prime
Internal
Molecular Weight (mw)
Extinction Coeficient (ec)
Technical Info (pdf)
Absorbance MAX
Emission MAX
Absorbance EC

26-6524
Fluorescent Dyes
[PBlue-455]
Y
Y
Y
339.21
30
PS26-6524.pdf
410
455
30

Catalog No	Scale	Price
26-6524-05	50 nmol	$378.00
26-6524-02	200 nmol	$378.00
26-6524-01	1 umol	$491.00
26-6524-03	2 umol	$737.00
26-6524-06	5 umol	$2,209.50
26-6524-10	10 umol	$3,931.00
26-6524-15	15 umol	$4,914.00

Related Modifications
AZDye-488-N	MarBlue-460 NHS	Cy3-5'
Fam (6-FAM)-5'	Cy5-5'	PBlue-455 NHS

Click here for a list of fluorophores.

This modification is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is required. A C3, C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

Yield of Post Synthesis NHS, Maleimide & Click Ligand Conjugation*
Oligo Scale of Synthesis	Yield, nmols
50 nmol	2 nmol
200 nmol	5 nmol
1 umol	16 nmol
2 umol	30 nmol
5 umol	75 nmol
10 umol	150 nmol
15 umol	225 nmol
* The yield will be lower for oligos longer than 50mer. Click here for yield table of long oligos.
* Click here for RNA Oligos scale of synthesis and yield.
NHS Ligand conjugation requires a primary amino group. Gene Link offers a wide selection of amino modifications for 5', 3' and internal sites. Click here for a list of conjugation chemistry modifications.
Maleimide Ligand conjugation requires a thiol group. Gene Link offers a wide selection of thiol modifications for 5', 3' and internal sites. Click here for a list of conjugation chemistry modifications.
Click Chemistry Ligand conjugation requires a corresponding Click modification; examples Alkyne:Azide, Azide:DBCO, BCN:Azide, BCN: TCO:Tetrazine. Gene Link offers a wide selection of click modifications for 5', 3' and internal sites. Click here for a list of click chemistry modifications.

PBlue-455 is a UV-excitable, bright blue fluorescent dye used for labeling oligonucleotides excitable by the 407 nm spectral line of blue diode (violet color) laser. PBlue-455 has an absorbance maximum of 410 nm and an emission maximum of 455 nm. Because UV light can photo damage labeled oligos, and many kinds of cells and tissues autofluoresce under UV light, PBlue-455 can only be used in a limited number of applications. Nevertheless, for such applications as nucleic acid microarrays and in situ hybridization, where a blue fluorescent probe provides a easily distinguishable, contrasting color to the green, yellow, orange and red fluorescence produced by longer-wavelength probes, PBlue-455 can be a good choice.

- PBlue-455 NHS