MarBlue-460NHS

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This modification is a post synthesis conjugation to a primary amino group thus an additional modification with an amino group is required. A C3, C6 or C12 amino group can be placed at the 5' or for the 3' end a C3 or C7 amino and for internal positions an amino modified base is used, e.g Amino dT C6.

MBlue-460 NHS is 6,8-Difluoro-7-hydroxy-4-methylcoumarin NHS Ester exhibiting bright blue fluorescence emission near 460 nm is optimally excited by the intense 365 nm spectra line of the mercury-arc lamp and detect optimally with DAPI optical filter sets. Because the pKa value of 6,8-Difluoro-7-hydroxy-4-methylcoumarin derivatives are 2-3 log units lower compared to those of the corresponding 7-hydroxycoumarin conjugates, 6,8-Difluoro-7-hydroxy-4-methylcoumarin conjugates are strongly fluorescent even at neutral pH.
MBlue-460 is an NHS and is available as a fluorescent dye that can be conjugated to an oligo, either RNA or DNA. This is a post synthesis conjugation to a primary amino group. The amino group can be placed at the 5' and 3' and for internal positions an amino modified base is used, e.g Amino dT C6